ABN1735

Anti-Oxytocin Receptor

from rabbit, purified by affinity chromatography

• 別名: Oxytocin receptor, OT-R
• UNSPSCコード: 12352203
• eCl@ss: 32160702

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: mouse, human
• テクニック: western blot: suitable
• NCBIアクセッション番号: NP_001074616
• UniProtアクセッション番号: P97926
• 輸送温度: ambient
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... OXTR(5021) ; mouse ... Oxtr(18430)

詳細

詳細

Oxytocin receptor (UniProt P97926; also known as OT-R) is encoded by the Oxtr (also known as Otr) gene (Gene ID 18430) in murine species. Oxytocin receptor (OT-R) is widely expressed in the reproductive tract, brain, kidneys, and many other organs, where it mediates the biological functions of oxytocin (OT) in mammals. Best known for their role in lactation and parturition, OT-R and OT meidate a wide variety of physiological functions, including estrous cycle, bone formation, social behaviors, as well as regulation of energy homeostasis, including food intake and thermoregulation. Central administration of OT decreases food consumption in rats, and nesfatin (a satiety hormone) increases OT secretion. Oxt-null and Oxtr-null mice display aberrant thermoregulatory function. In addition, Oxtr-knockout mice have a morphological defect in brown adipose tissue (BAT) cells and develop obesity. Human and murine oxytocin receptors are 7-transmembrane (a.a. 39-63, 75-97, 114-135, 155-175, 203-225, 275-293, 309-331) GPCRs with their N-terminal end at the extracellular side (a.a. 1-38) and C-terminal end at the cytoplasmic side (human a.a. 332-389 and mouse a.a. 332-388).

特異性

This polyclonal antibody specifically stained wild-type, but not Oxtr-knockout, mouse brain tissue sections (Courtesy of Dr. Moses V. Chao, Langone Medical Center, New York University, U.S.A).

免疫原

BSA-conjugated linear peptide corresponding to a sequence from mouse oxytocin receptor N-terminal extracellular domain.

アプリケーション

Research Category
ニューロサイエンス

Anti-Oxytocin Receptor Antibody, Cat. No. ABN1735, is a highly specific rabbit polyclonal antibody, that targets Oxytocin Receptor 1 and has been tested in Western Blotting.

Western Blotting Analysis: A 1:500 dilution from a representative lot detected oxytocin receptor in 10 µg of A431, HEK293, HeLa, Jurkat, MCF-7, and Raji membrane extracts.

Immunofluorescence Analysis: A 1:1000 dilution from a representative lot detected oxytocin receptor immunoreactivity in wild-type, but not Oxtr-knockout, mouse frozen brain tissue sections (Courtesy of Dr. Moses V. Chao, Langone Medical Center, New York University, U.S.A).

品質

Evaluated by Western Blotting in human uterus.

Western Blotting Analysis: A 1:500 dilution of this antibody detected oxytocin receptor in 10 µg of human uterus tissue lysate.

ターゲットの説明

~55-60 kDa observed. The target band size appears larger than the calculated molecular weight of 42.77/42.81 kDa (human/mouse) due to glycosylation. Uncharacterized bands may be observed in some lysate(s).

物理的形状

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% sodium azide

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable