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由来生物
rabbit
品質水準
抗体製品の状態
serum
抗体製品タイプ
primary antibodies
クローン
polyclonal
化学種の反応性
mouse
テクニック
ChIP: suitable
immunohistochemistry: suitable
western blot: suitable
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
dry ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
mouse ... Jarid2(16468)
関連するカテゴリー
詳細
Protein Jumonji (Jarid2), also known as Jumonji/ARID domain-containing protein 2, and encoded by the gene name Jarid2 is a member of the JARID2 family. Jarid 2 is a regulator of histone methyltransferase complexes, and therefore plays an essential role in embryonic development, including heart and liver development, neural tube fusion process and hematopoiesis. Recent studies have shown that Jarid 2 modulates histone methyltransferase activity and promotes the recruitment of histone methyltransferase complexes to their target genes. However, Jarid 2 does not have histone demethylase activity. Jarid 2 binds DNA and mediates the recruitment of the PRC2 complex to target genes in embryonic stem cells. In embryonic stem cells, Jarid 2 associates with the PRC2 complex and inhibits trimethylation of ′Lys-27′ of histone H3 (H3K27me3) by the PRC2 complex, thereby playing a key role in differentiation of embryonic stem cells and normal development. In cardiac cells, Jarid 2 is required to repress expression of cyclin-D1 (CCND1) by activating methylation of ′Lys-9′ of histone H3 (H3K9me) by the GLP1/EHMT1 and G9a/EHMT2 histone methyltransferases. Jarid 2 has been implied to act as a transcriptional repressor of ANF via its interaction with GATA4 and NKX2-5. In addition, Jarid 2 might participate in the negative regulation of cell proliferation signaling.
免疫原
GST-tagged recombinant protein corresponding to mouse Jarid2.
アプリケーション
Research Category
エピジェネティクス及び核内機能分子
エピジェネティクス及び核内機能分子
Research Sub Category
エピジェネティクス
エピジェネティクス
Anti-Jarid2 is an antibody against Jarid2 for use in WB, ChIP;IH.
Western Blotting Analysis: A representative lot of this antibody detected Jarid2 in mouse embryo extracts at E12.5.
Immunoprecipitation Analysis: A representative lot of this antibody immunopreciptated Jarid2 from HEK293 transfected cell lysates (Mysliwiec, M.R., et al. (2007) FEBS. 581(14):2633-2640).
Chromatin Immunoprecipitation Analysis (ChIP-chip): A representative lot of this antibody immunopreciptated Jarid2 using chromatin from E17.5 Mouse heart tissue lysate using ChIP-chip (Mysliwiec, M.R., et al. (2011) JBC. 287(2):1235-1241).
Immunohistochemistry Analysis: A representative lot of this antibody detected Jarid2 in E17.5 Mouse heart tissue sections (Mysliwiec, M.R., et al. (2011) JBC. 287(2):1235-1241).
Immunoprecipitation Analysis: A representative lot of this antibody immunopreciptated Jarid2 from HEK293 transfected cell lysates (Mysliwiec, M.R., et al. (2007) FEBS. 581(14):2633-2640).
Chromatin Immunoprecipitation Analysis (ChIP-chip): A representative lot of this antibody immunopreciptated Jarid2 using chromatin from E17.5 Mouse heart tissue lysate using ChIP-chip (Mysliwiec, M.R., et al. (2011) JBC. 287(2):1235-1241).
Immunohistochemistry Analysis: A representative lot of this antibody detected Jarid2 in E17.5 Mouse heart tissue sections (Mysliwiec, M.R., et al. (2011) JBC. 287(2):1235-1241).
品質
Evaluated by Western Blotting using recombinant protein GST-Jarid2 (aa529-798).
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected 0.2 µg of recombinant protein GST-Jarid2 (aa529-798).
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected 0.2 µg of recombinant protein GST-Jarid2 (aa529-798).
ターゲットの説明
~57 kDa observed from recombinant protein.
Endogenous Jarid2 protein has a calculated molecular weight of 137 kDa
Uncharacterized bands may be observed in some lysates.
Endogenous Jarid2 protein has a calculated molecular weight of 137 kDa
Uncharacterized bands may be observed in some lysates.
物理的形状
Unpurified
Rabbit polyclonal serum in buffer containing 0.05% sodium azide.
保管および安定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
その他情報
Concentration: Please refer to lot specific datasheet.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
ABE425:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Characterization of zinc finger protein 496 that interacts with Jumonji/Jarid2.
Mysliwiec, MR; Kim, TG; Lee, Y
Febs Letters null
Jarid2 (Jumonji, AT rich interactive domain 2) regulates NOTCH1 expression via histone modification in the developing heart.
Mysliwiec, MR; Carlson, CD; Tietjen, J; Hung, H; Ansari, AZ; Lee, Y
The Journal of Biological Chemistry null
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