ABE1408

Anti-DNA polymerase beta Antibody

from rabbit, purified by affinity chromatography

• 別名: DNA polymerase beta, DNA polymerase beta
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: rat, human, mouse
• 化学種の反応性（ホモロジーによる予測）: canine (based on 100% sequence homology), Xenopus (based on 100% sequence homology), bovine (based on 100% sequence homology), feline (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology)
• テクニック: immunohistochemistry: suitable; western blot: suitable
• NCBIアクセッション番号: NP_002681
• UniProtアクセッション番号: P06746
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: bovine ... Polb(614688) ; cat ... Polb(101099797) ; dog ... Polb(494001) ; frog ... Polb(448741) ; human ... POLB(5423) ; mouse ... Polb(18970) ; rat ... Polb(29240) ; rhesus monkey ... Polb(704801)

詳細

詳細

DNA polymerase beta (EC 2.7.7.7; UniProt P06746; also known as DNA pol beta, DNA polymerase beta subunit) is encoded by the POLB gene (Gene ID 5423) in human. DNA polymerase beta is involved in DNA recombination and base-excision repair (BER). Upon oxidative DNA damage, Polb translocates to the nucleus, where it associates with AP endonuclease 1 (Apex) to carry out DNA repair. Apex makes a single nick 5′ to an abasic site in double-stranded DNA, Polb then inserts the replacement nucleotide(s). Aberrant POLB overexpression results in an increased rate of spontaneous mutagenesis and a highly mutagenic tolerance phenotype. Thirty percent of all tumors are reported to harbor mutations in the POLB gene.

免疫原

KLH-conjugated linear peptide corresponding to the C-terminus of human DNA polymerase beta.

アプリケーション

Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected DNA polymerase beta in normal rat testis, normal human kidney, as well as human lung & prostate cancer tissue sections.

This Anti-DNA polymerase beta Antibody is validated for use in Western Blotting, Immunohistochemistry for the detection of DNA polymerase beta.

品質

Evaluated by Western Blotting in human testis tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected DNA polymerase beta in 10 µg of human and mouse testis tissue, Jurkat, and HeLa lysates.

ターゲットの説明

~38 kDa observed. Uncharacterized band(s) may appear in some lysates.

その他情報

Concentration: Please refer to lot specific datasheet.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable