ABE1029

Anti-acetyl Histone H3 Antibody (Lys122)

from rabbit, purified by affinity chromatography

• 別名: Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/I, Histone H3/j, Histone H3/k, Histone H3/l
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• クローン: polyclonal
• application: DB; ICC; IP; WB
• 化学種の反応性: mouse, human
• テクニック: dot blot: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: mouse, human
• テクニック: dot blot: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• NCBIアクセッション番号: NP_003520
• UniProtアクセッション番号: P68431
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: acetylation (pLys122)
• 遺伝子情報: human ... HIST1H3F(8968)

詳細

詳細

Histone H3, also known as Histone H3.1t (H3/t), H3t, H3/g, and encoded by the gene name HIST3H3/ H3FT, is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Therefore, histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. High levels of phosphorylation of Histone H3 are associated with mitosis.

免疫原

Epitope: Near C-terminal

KLH-conjugated linear peptide corresponding to human acetyl Histone H3 (Lys122) near the C-terminal.

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

Research Sub Category
核受容体

Anti-acetyl Histone H3 (Lys122) is an antibody against acetyl Histone H3 (Lys122) for use in WB, ICC, DB, IP.

Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected acetyl Histone H3 (Lys122) in HeLa and A431cells.
Dot Blot Analysis: A 1:1,000 dilution from a representative lot detected acetyl Histone H3 (Lys122) in Histone peptides with various modifications.
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated acetyl Histone H3 (Lys122) in 500 µg of sodium butyrate treated HeLa acid extract.

品質

Evaluated by Western Blotting in sodium butyrate treated HeLa acid extract.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected acetyl Histone H3 (Lys122) in 10 µg of sodium butyrate treated HeLa acid extract.

ターゲットの説明

~17 kDa observed

物理的形状

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable