AB5935

Anti-Tyrosine Hydroxylase Antibody, phosphoSer 40

Chemicon^®, from rabbit

• 別名: Tyrosine Monooxygenase
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: avian, rat, mouse
• 化学種の反応性（ホモロジーによる予測）: quail (100% sequence homology), nonhuman primates (100% sequence homology), human (100% sequence homology), porcine (100% sequence homology)
• メーカー／製品名: Chemicon^®
• テクニック: immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• NCBIアクセッション番号: NM_000360.3
• UniProtアクセッション番号: P07101
• 輸送温度: dry ice
• ターゲットの翻訳後修飾: phosphorylation (pSer40)
• 遺伝子情報: human ... TH(7054) ; rat ... Th(25085)

詳細

詳細

Tyrosine hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.

特異性

Other species have not been tested.

Tyrosine Hydroxylase, phosphoSer40. The antibody recognizes a protein of 60 kDa corresponding to TH phosphorylated at Ser40. Depending on brain region being studied, protein loads and detection methods used some higher molecular weight bands may be detected.

免疫原

Epitope: phospho-Ser40

Synthetic peptide from the phosphoSer40 of rat Tyrosine Hydroxylase.

アプリケーション

Research Category
ニューロサイエンス

Research Sub Category
神経伝達物質及び受容体

Anti-Tyrosine Hydroxylase Antibody, phosphoSer 40 detects level of Tyrosine Hydroxylase & has been published & validated for use in IC, IH & WB.

Immunohistochemistry:
A 1:1,000 dilution of a previous lot was used in IH.

Immunocytochemistry:
A 1:1,000 dilution of a previous lot was used in IC.

Optimal working dilutions must be determined by the end user.

品質

Routinely evaluated by Western Blot on PC12 lysates.

Western Blot Analysis:
1:500 dilution of this lot detected TYROSINE HYDROX PSER40 on 10 μg of PC12 lysates.

ターゲットの説明

60 kDa

物理的形状

ImmunoAffinity Purified

Purified rabbit polyclonal liquid in buffer containing 10mM Hepes, pH 7.5, 150mM NaCl, 100 µg/mL BSA, and 50% glycerol.

保管および安定性

Stable for 6 months at -20°C in undiluted from date of receipt.

アナリシスノート

Control
POSITIVE CONTROL: brain (sympathetic nerve terminals) and adrenal glands.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2