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由来生物
rabbit
品質水準
抗体製品の状態
affinity purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
polyclonal
精製方法
affinity chromatography
化学種の反応性
rat
メーカー／製品名
Chemicon®
テクニック
immunocytochemistry: suitable
immunohistochemistry: suitable (tissue)
immunoprecipitation (IP): suitable
western blot: suitable
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
rat ... Cacna1B(257648)
特異性
Recognizes a 1B subunit (both LMW and HMW forms) of voltage-gated calcium channel. Does not cross react with any other calcium channel antigens tested so far.
SPECIES REACTIVITIES: It is expected that the antibody will also work on mouse due to sequence homology (15/17). Other species have not been tested.
SPECIES REACTIVITIES: It is expected that the antibody will also work on mouse due to sequence homology (15/17). Other species have not been tested.
免疫原
Purified peptide CNB1 from 851-867 of alpha 1B subunit of rat brain voltage-gated calcium channel (Accession Q02294; Westenbroek et al. 1992) containing N-terminal lysine and tyrosine, conjugated to KLH with gluteraldehyde (Harlow & Lane 1988).
アプリケーション
Research Category
ニューロサイエンス
ニューロサイエンス
Research Sub Category
イオンチャネル及びトランスポーター
神経炎症及び疼痛
イオンチャネル及びトランスポーター
神経炎症及び疼痛
Detect Cav2.2 using this Anti-Cav2.2 Antibody validated for use in IC, IP & WB.
In preparing full-length protein samples for Western blot or immunoprecipitation, the procedures should be performed at 4°C, with the following protease inhibitor mixture: pepstatin A (1 μg/mL), leupeptin (1 μg/mL), aprotinin (1 μg/mL), Pefabloc SC (0.2 mM), benzamidine (0.1 mg/mL), and calpain inhibitors I and II (8 μg/mL each).
Immunoprecipitation
Immunocytochemistry. Recommend subsequent staining with a labeled second antibody.
Western blot: 1.5 to 3.0 μg/mL (1:100-1:200) using ECL
Dilutions should be made using a carrier protein such as BSA (1-3%)
Optimal working dilutions must be determined by the end user.
Immunoprecipitation
Immunocytochemistry. Recommend subsequent staining with a labeled second antibody.
Western blot: 1.5 to 3.0 μg/mL (1:100-1:200) using ECL
Dilutions should be made using a carrier protein such as BSA (1-3%)
Optimal working dilutions must be determined by the end user.
物理的形状
Affinity purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA and 0.05% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
保管および安定性
Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at 2-8°C in undiluted aliquots for up to 2 weeks. For longer periods, it is recommended to add glycerol (ACS grade or better) at a ratio of 1:1 and store the antibody at -20°C for up to 6 months. Avoid repeated freeze/thaw cycles.
アナリシスノート
Control
Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 10 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 10 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
毒物及び劇物取締法
毒物
Jan Code
AB5154-200UL:
AB5154-50UL:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
J H Xu et al.
Neuropathology and applied neurobiology, 36(1), 71-85 (2009-10-09)
To investigate the subcellular localization of Ca(v)2.2 calcium channel in the mouse central nervous system (CNS), and changes of Ca(v)2.2 at acute and chronic stages during and after pilocarpine-induced status epilepticus (PISE), in order to find out the roles it
Kang Zhang et al.
Frontiers in pharmacology, 8, 302-302 (2017-06-13)
The sigma-1 receptor is a 223 amino acids molecular chaperone with a single transmembrane domain. It is resident to eukaryotic mitochondrial-associated endoplasmic reticulum and plasma membranes. By chaperone-mediated interactions with ion channels, G-protein coupled receptors and cell-signaling molecules, the sigma-1
R E Westenbroek et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 12(6), 2259-2267 (1992-06-01)
Type I and type III Na+ channels are localized mainly in neuronal cell bodies in mouse brain. Type II channels are preferentially localized in unmyelinated fiber tracts but are not detectable in normally myelinated fibers. In shiverer mice, which lack
Hang Zhou et al.
Molecular brain, 8, 42-42 (2015-07-18)
The density of functional synapses is an important parameter in determining the efficacy of synaptic transmission. However, how functional presynaptic terminal density is regulated under natural physiological conditions is still poorly understood. We studied the factors controlling the density of presynaptic
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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