AB3098

Anti-Orexin-A Antibody

Chemicon^®, from rabbit

• 別名: Anti-NRCLP1, Anti-OX, Anti-PPOX
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: feline, rat, human, mouse
• メーカー／製品名: Chemicon^®
• テクニック: ELISA: suitable; western blot: suitable
• NCBIアクセッション番号: NM_001524.1
• UniProtアクセッション番号: O43612
• 輸送温度: dry ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... PPOX(5498)

詳細

特異性

Mouse Orexin-A. Several peptides associated with feeding behavior have been reported recently. Orexins (Orexin-A and Orexin-B) are a family of hypothalamic neuropeptides selectively expressed in the hypothalamus (Sakurai et al. 1998). Orexin-A and Orexin-B are derived from the same precursor (Prepro-orexin) by proteolytic cleavage. Prepro-orexin is an 130 aa peptide with a putative 33 aa secretory sequence, a hydrophobic core followed by residues with small polar side chains. The expression was detected in brain and to a small extent in testis (Sakurai et al. 1998). These neuropeptides bind and activate two closely related Orexin receptors--G protein coupled receptors (GPCRs) OX1R and OX2R.

免疫原

An 18 amino acid peptide mapping near the amino terminus of mouse Orexin-A. The immunogen sequence is identical in human, rat, porcine, ovine, bovine and canine and has 81% homology with chicken.

アプリケーション

Research Category
ニューロサイエンス

Research Sub Category
CNSによる代謝制御

サーカディアンリズム及び睡眠

This Anti-Orexin-A Antibody is validated for use in ELISA, WB for the detection of Orexin-A.

Western blot: 1-10 μg/mL using Chemiluminescence technique ELISA: 1:10,000 - 100,000 using 5-100 ng of control peptide per well. NOTE: Orexin A is a 3.5kDa protein. Adjust your SDS-PAGE gel and lane size markers accordingly.

Immunohistochemistry: Frozen 4% PFA tissue with ABC detection {Wagner et al 2000}.

Optimal working dilutions must be determined by end user.

物理的形状

Liquid in PBS, pH 7.4 with 0.1% BSA and 0.1% sodium azide.

保管および安定性

Maintain frozen at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable