AB16969

Anti-Caspase 9 Antibody, proform & lg. subunit

Chemicon^®, from rabbit

• 別名: ICE-LAP6, Mch-6, Apaf-3
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 化学種の反応性: mouse, human
• 以下との反応禁止: rat
• メーカー／製品名: Chemicon^®
• テクニック: western blot: suitable
• NCBIアクセッション番号: NM_001229.2; NM_032996.1
• UniProtアクセッション番号: P55211
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... CASP9(842)

詳細

特異性

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3 (Duan et al. 1996; Srinivasula et al. 1996; Nijhawan et al. 1997). Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation (Nijhawan et al. 1997). Caspase-9 is also activated by granzyme B and CPP32 (Duan et al. 1996; Srinivasula et al. 1996). Activated caspase-9 cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis (Nijhawan et al. 1997). Caspase-9 plays a central role in cell death induced by a wide variety of apoptosis inducers including TNF-alpha, TRAIL, anti-CD-95, FADD, and TRADD (Pan et al. 1998). Caspase-9 is expressed in a variety of human tissues (Duan et al. 1996; Srinivasula et al. 1996).

免疫原

Epitope: proform & lg. subunit

Synthetic peptide corresponding to amino acids 41-56 of human caspase-9 (Duan et al. 1996; Srinivasula et al. 1996; Nijhawan et al. 1997). Recognizes proform (46 kDa); sequence analysis predicts cross-reactivity with mouse caspase-9 (>90%).

アプリケーション

Research Category
アポトーシス及び癌

代謝

Research Sub Category
カスパーゼ

酵素及び生化学

Detect Caspase 9 using this Anti-Caspase 9 Antibody, proform & lg. subunit validated for use in WB.

Western blot: 1:1,000 to 1:2,000

Whole cell lysate from HeLa cells can be used as a positive control and a 46 kDa band should be detected.

Optimal working dilutions must be determined by end user.

関連事項

Replaces: 04-443

物理的形状

Format: Purified

Immunoaffinity chromatography purified immunoglobulin. Liquid in PBS, containing 0.02% sodium azide.

保管および安定性

The undiluted antibody solution is stable for approximately 6 months when stored 2-8°C.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable