654205-M

TNF-α, Human, Recombinant, E. coli

• 別名: TNF-α, Human, Recombinant, E. coli, rhTNF-α, rh-Cachectin, Tumor Necrosis Factor-α
• CAS番号: 94948-59-1
• MDL番号: MFCD00148692
• UNSPSCコード: 12352202
• NACRES: NA.27

特性

• 由来生物: human
• 品質水準: 100
• リコンビナント: expressed in E. coli
• アッセイ: ≥95% (SDS-PAGE)
• 形状: lyophilized
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze
• 不純物: ≤0.1 ng/μg Endotoxin (ng/μg TNF-α)
• 溶解性: distilled water: 0.1—1 mg/mL
• 保管温度: −70°C
• 遺伝子情報: human ... TNF(7124)

詳細

詳細

Research area: Apoptosis

Tumor Necrosis Factor alpha (TNF α), a homotrimer protein made of 157 amino acids, is produced by macrophages/monocytes, T-lymphocytes, and natural killer cells during acute inflammation. soluble and transmembrane form. The precursor form of TNF-α is in transmembrane form (tmTNF-α), which is processed to release soluble form (sTNF-α).

アプリケーション

TNF-α, Human, Recombinant, E. coli has been used to stimulate NF-κB activation and study the effects of pancreatic elastase on cellular responses.

生物化学的／生理学的作用

Recombinant, human tumor necrosis factor-α expressed in E. coli., a cytokine produced primarily by activated macrophages, activates a variety of immune defense mechanisms by interactions with polymorphonuclear leukocytes, T cells, antibody-producing B lymphocytes, fibroblasts, and hematopoietic bone marrow cells. Activity is not species-specific. Induces apoptosis in human blood and bone marrow neutrophils and in endothelial cells. Increases the iNOS levels in vascular smooth muscle cells. Involved in pathophysiological processes of several chronic and acute diseases. Stimulates stress-activated protein kinase (SAPK). ED₅₀ = 20-50 pg/ml as measured in a cytotoxicity assay with the TNF-α-susceptible murine L-929 cell line in the presence of Actinomycin D (Cat. No. 114666)

Tumor Necrosis Factor alpha (TNF alpha), is an inflammatory cytokine. It coordinates a range of signaling events within cells, leading to necrosis or apoptosis. TNF-α initiates signal transduction pathways by binding to TNFR-1 and TNFR-2. These pathways cause cellular processes such as cell survival, differentiation, and proliferation.

警告

Toxicity: Standard Handling (A)

再構成

Following reconstitution, aliquot and freeze (-20°C or -70°C) for long-term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at -20°C or -70°C or up to 1 month at 4°C.

Reconstitute with sterile, dH₂O to a final concentration of 0.1-1 mg/ml. Further dilutions should be made in low endotoxin medium or aqueous buffer containing carrier protein such as heat inactivated FCS or tissue culture grade BSA.

その他情報

Merck Index 13, 9882.
Tsuchida, H., et al. 1995. J. Immunol. 154, 2403.
Westwick, J.K., et al. 1995. J. Biol. Chem. 270, 22689.
Polunovsky, V.A., et al. 1994. Exp. Cell Res.214, 584.
Koide, M., et al. 1993. FEBS Lett.318, 213.
Vilcek, J., and Lee, T.H. 1991. J. Biol. Chem.266, 7313.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable