616403

TACE Substrate III, Fluorogenic

A fluorescence resonance energy transfer (FRET) peptide substrate for TACE (Km = 19 µM).

• 別名: TACE Substrate III, Fluorogenic, TNF-α Convertase Enzyme Substrate II, Fluorogenic, ADAM-17 Substrate II, Fluorogenic, DABCYL-LAQAVRSSSR-EDANS, TNF-α Convertase Enzyme Substrate II, Fluorogenic, DABCYL-LAQAVRSSSR-EDANS, ADAM-17 Substrate II, Fluorogenic
• 実験式（ヒル表記法）: C₇₀H₁₀₄N₂₂O₁₈S
• CAS番号: 396716-14-6
• 分子量: 1573.78
• UNSPSCコード: 12352200

特性

• 品質水準: 100
• アッセイ: ≥95% (HPLC)
• フォーム: lyophilized solid
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; protect from light
• 色: red
• 溶解性: 50% acetic acid: 5 mg/mL; DMSO: 5 mg/mL
• 蛍光検出: λ_ex ~340 nm; λ_em ~490 nm
• 輸送温度: ambient
• 保管温度: −20°C

詳細

詳細

A fluorescence energy transfer (FRET) peptide substrate for tumor necrosis factor (TNF)-α convertase (TACE) (K_m ~19 µM). Cleavage occurs between the alanine and valine residues. Useful for screening TACE inhibitors. Excitation max: ~340 nm; emission max: ~490 nm.

A fluorescence resonance energy transfer (FRET) peptide substrate for TACE (K_m = 19 µM). Substrate cleavage occurs at the Ala-Val bond. Useful for the screening of TACE and SARS protease 3-CL^PRO inhibitors.

生物化学的／生理学的作用

Cell permeable: no

Km = 19 µM as a fluorescence resonance energy transfer (FRET) peptide substrate for TACE

Primary Target
A fluorescence resonance energy transfer (FRET) peptide substrate for TACE

Product does not compete with ATP.

Reversible: no

包装

Yes

警告

Toxicity: Standard Handling (A)

シーケンス

DABCYL-Leu-Ala-Gln-Ala-Val-Arg-Ser-Ser-Ser-Arg-EDANS

物理的形状

Supplied as a trifluoroacetate salt.

再構成

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

その他情報

Bacha, U., et al. 2004. Biochemistry43, 4906.
Patel, I.R., et al. 1998. J. Immunol.160, 4570.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable