616376

TRAIL, Human, Recombinant, NSO cells

• 別名: Apo2L; TNF-Related Apoptosis-Inducing Ligand
• UNSPSCコード: 12352202
• NACRES: NA.77

特性

• リコンビナント: expressed in NSO cells
• 品質水準: 100
• アッセイ: ≥97% (SDS-PAGE)
• フォーム: lyophilized
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze
• 不純物: ≤1 EU/μg Endotoxin (EU/μg TRAIL)
• 輸送温度: ambient
• 保管温度: −70°C

詳細

詳細

Recombinant, human TRAIL (amino acids 95-281) with an amino-terminal 6X histidine tag and expressed in NSO cells. A type II transmembrane protein with a C-terminal extracellular domain that exhibits homology to other members of this tumor necrosis factor (TNF) family. Displays potent anti-tumor activity against selected targets. Induces apoptosis in a number of transformed cell lines but not in normal tissues. Recombinant bioactive TRAIL is a non-disulfide-linked homotrimer but may contain a small amount (up to 10%) of disulfide-linked homodimers.

Recombinant, human TRAIL expressed in NSO cells a type II transmembrane protein with a C-terminal extracellular domain, which exhibits homology to other TNF family members. Displays potent antitumor activity against selected targets. Induces apoptosis in a number of transformed cell lines but not in normal tissues.

生物化学的／生理学的作用

ED₅₀ = 4-12 ng/ml as measured by its ability to induce cytotoxicity in mouse L-929 cell line in the presence of metabolic inhibitors actinomycin D

警告

Toxicity: Standard Handling (A)

物理的形状

Lyophilized from sterile-filtered PBS, 50 µg BSA/µg TRAIL, pH 7.4.

再構成

Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C or -70°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or up to 3 months at -20°C or -70°C. Avoid freeze/thaw cycles of solutions.

Reconstitute to ≥20 µg/ml in sterile PBS, 0.1% BSA.

その他情報

Griffith, T.S., et al. 1999. J. Exp. Med.189, 1343.
Mariani, S.M., et al. 1997. J. Cell Biol.137, 221.
Pan, G., et al. 1997. Science277, 815.
Pan, G., et al. 1997. Science276, 111.
Sheridan, J.P., et al. 1997. Science277, 818.
Pitti, R.M., et al. 1996. J. Biol. Chem.271, 12687.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable