565758

β-Secretase Substrate IV, Fluorogenic

≥95% (HPLC), lyophilized solid, β-secretase substrate, Calbiochem^®

• 別名: β-Secretase Substrate IV, Fluorogenic, FS-1, H-RE(EDANS)EVNLDAEFK(DABCYL)R-OH, Memapsin 2 Substrate I, Fluorogenic
• 実験式（ヒル表記法）: C₉₁H₁₂₉N₂₅O₂₅S
• CAS番号: 310427-94-2
• 分子量: 2005.22
• UNSPSCコード: 12352202
• NACRES: NA.77

特性

• 製品名: β-Secretase Substrate IV, Fluorogenic, A highly sensitive fluorescence resonance energy transfer (FRET) peptide substrate, based on the EVNLDAEF sequence derived from the β-secretase site of the Swedish mutation of Amyloid Precursor Protein (APP).
• 品質水準: 100
• アッセイ: ≥95% (HPLC)
• フォーム: lyophilized solid
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; protect from light
• 色: red
• 溶解性: DMSO: 5 mg/mL
• 蛍光検出: λ_ex ~350 nm; λ_em ~490 nm
• 輸送温度: ambient
• 保管温度: −20°C

詳細

詳細

A highly sensitive fluorescence resonance energy transfer (FRET) peptide substrate (excitation max.: ~350 nm; emission max.: ~490), based on the GVNLDAEF sequence derived from the β-secretase site of the Swedish mutation of Amyloid Precursor Protein. Hydrolysis of the substrate at Leu-Asp bond by β-secretase results in fluorescence enhancement. Reported K_cat/k_m values (min^-1/µM) for pro-mempasin 2 and its proteolytically activated species Leu^28p-mempasin 2 are 0.044 and 0.040, respectively (at pH 4.5, 37°C).

A highly sensitive fluorescence resonance energy transfer (FRET) peptide substrate, based on the EVNLDAEF sequence derived from the β-secretase site of the Swedish mutation of Amyloid Precursor Protein (APP). Hydrolysis of the substrate (Leu-Asp cleavage) by β-secretase results in fluorescence enhancement. k_cat/K_m values (min^-1/µM) at 37°C, pH 4.5, for pro-memapsin 2 and its activated species Leu^28p-memapsin 2 are 0.044 and 0.040, respectively.

生物化学的／生理学的作用

Cell permeable: no

Primary Target
β-secretase substrate

Product does not compete with ATP.

Reversible: no

包装

Packaged under inert gas

警告

Toxicity: Standard Handling (A)

シーケンス

H-Arg-Glu(EDANS)Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Lys(DABCYL)Arg-OH

物理的形状

Supplied as a trifluoroacetate salt.

再構成

Following reconstitution, aliquot and freeze at -20°C. Stock solutions are stable for up to 1 month at -20°C.

その他情報

Ermolieff, J., et al. 2000. Biochemistry 39, 12450.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable