524390

Phorbol-12,13-dibutyrate

Strong irritant for mouse skin, but only moderately active as a tumor promoter.

• 別名: Phorbol-12,13-dibutyrate, PDBu, PKC Activator II
• 実験式（ヒル表記法）: C₂₈H₄₀O₈
• CAS番号: 37558-16-0
• 分子量: 504.61
• MDL番号: MFCD00036783
• UNSPSCコード: 12352211

特性

• 品質水準: 100
• アッセイ: ≥99% (HPLC)
• 形状: solid (crystalline)
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze
• 色: clear
• 溶解性: DMSO: 10 mg/mL
• 輸送温度: ambient
• 保管温度: −20°C
• InChI: 1S/C28H40O8/c1-7-9-20(30)35-24-16(4)27(34)18(22-25(5,6)28(22,24)36-21(31)10-8-2)12-17(14-29)13-26(33)19(27)11-15(3)23(26)32/h11-12,16,18-19,22,24,29,33-34H,7-10,13-14H2,1-6H3/t16-,18+,19-,22-,24-,26-,27-,28-/m1/s1
• InChI Key: BQJRUJTZSGYBEZ-YVQNUNKESA-N

詳細

詳細

Strong irritant for mouse skin, but only moderately active as a tumor promoter. Activates protein kinase C (PKC). Stimulates the phosphorylation of Na⁺-K⁺ ATPase, thereby inhibiting its activity. Promotes the expression of iNOS in cultured hepatocytes. PDBu can be diluted to 30 µM in aqueous solutions, so it is commonly used in binding studies or in applications requiring high concentrations. It is easier to remove PDBu than PMA from cells in culture.

Strong irritant for mouse skin, but only moderately active as a tumor promoter. Activates protein kinase C. Stimulates the phosphorylation of Na⁺,K⁺- ATPase, thereby inhibiting its activity. Promotes the expression of inducible NOS in cultured hepatocytes. Commonly used in binding studies or in applications requiring high concentrations of phorbol compounds.

生物化学的／生理学的作用

Cell permeable: no

Primary Target
Na⁺,K⁺- ATPase

Product does not compete with ATP.

Reversible: no

包装

Packaged under inert gas

警告

Toxicity: Highly Toxic & Carcinogenic / Teratogenic (I)

調製ノート

Further dilute with aqueous buffers just prior to use.

再構成

Following reconstitution, aliquot and freeze (-20°C). DMSO stock solutions are stable for up to 6 months at -20°C. With frequent use, the DMSO stock solutions may accumulate water. The presence of water in the stock solution will accelerate the decomposition of the product, resulting in significant loss of activity in 2-3 months.

その他情報

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Fisone, G., et al. 1995. J. Biol. Chem. 270, 2427.
Lee, E.J., et al. 1994. Brain Res. 665, 127.
Critz, S.D., et al. 1993. J. Neurochem.60, 1175.
Iredale, P.A., et al. 1993. Biochem. Pharmacol.45, 611.
Critz, S.D., and Byrne J.H. 1992. J. Neurophys.68, 1079.
Hortelano, S., et al. 1992. J. Biol. Chem. 267, 24937.
Ward C.A., and Moffat, M.P. 1992. J. Mol. Cell. Cardiol.24, 937.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• ピクトグラム: GHS05,GHS06,GHS08
• シグナルワード: Danger
• 危険有害性情報: H300 + H310 + H330 - H314 - H317 - H334 - H351
• 注意書き: P202 - P260 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• 危険有害性の分類: Acute Tox. 1 Dermal - Acute Tox. 1 Inhalation - Acute Tox. 2 Oral - Carc. 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1
• 保管分類コード: 6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable