5.08838

Puromycin, Dihydrochloride, Sterile-Filtered Aqueous Solution, Cell Culture-Tested

• 別名: Puromycin, Dihydrochloride, Sterile-Filtered Aqueous Solution, Cell Culture-Tested, (2 S)-2-Amino-N-((2 S,3 S,4 R,5 R)-5-(6-(dimethylamino)purin-9-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl)-3-(4-methoxyphenyl)
• 実験式（ヒル表記法）: C₂₂H₂₉N₇O₅ · 2HCl
• CAS番号: 58-58-2
• 分子量: 544.43
• MDL番号: MFCD00012691
• UNSPSCコード: 12352101

特性

• アッセイ: ≥98% (HPLC)
• 品質水準: 200
• フォーム: liquid
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; avoid repeated freeze/thaw cycles; protect from light
• 不純物: ≤10 EU/mg Endotoxin (dry weight)
• 保管温度: −20°C
• InChI: 1S/C22H29N7O5.2ClH/c1-28(2)19-17-20(25-10-24-19)29(11-26-17)22-18(31)16(15(9-30)34-22)27-21(32)14(23)8-12-4-6-13(33-3)7-5-12;;/h4-7,10-11,14-16,18,22,30-31H,8-9,23H2,1-3H3,(H,27,32);2*1H/t14-,15+,16?,18+,22+;;/m0../s1
• InChI Key: MKSVFGKWZLUTTO-USYAMCSGSA-N

詳細

詳細

An aminonucleoside antibiotic that acts as a prokaryotic and eukaryotic protein synthesis inhibitor.

An aminonucleoside antibiotic that acts as a prokaryotic and eukaryotic protein synthesis inhibitor. Resembles the aminoacyl-adenylyl terminus of aminoacyl-tRNA and competes for binding to the A site of the large ribosomal subunit. Its incorporation into a growing polypeptide results in termination of chain elongation and release of the nascent polypeptidyl-puromycin. Useful for selection of mammalian cell lines expressing a transfected pac gene (Streptomyces alboniger), whose product puromycin acetyltransferase, inactivates the drug via acetylation. Induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells. Recommended range for selection of transfected mammalian cells ranges from 1 to 30 µg/ml.

生物化学的／生理学的作用

Primary Target
RNA

Reversible: yes

警告

Toxicity: Standard Handling (A)

物理的形状

Supplied at 10 mg/ml in H₂O.

再構成

Following initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 6 months at -20°C.

その他情報

Chow, S.C., et al. 1995. Exp. Cell Res.216, 149.
Kaufman, S.H., et al. 1993. Cancer Res.53, 3976.
de la Luna, S. and Ortin, J. 1992. Methods Enzymol.216, 376.
Chow, S.C., et al. 1991. Exp. Cell Res.216, 149.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(℃): Not applicable