454578

Anti-Pan-NMDA NR1 Mouse mAb (R1JHL)

lyophilized, clone R1JHL, Calbiochem^®

• UNSPSCコード: 12352203
• NACRES: NA.43

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: culture supernatant
• 抗体製品タイプ: primary antibodies
• クローン: R1JHL, monoclonal
• フォーム: lyophilized
• 含まれません: preservative
• 交差性: rat
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze
• アイソタイプ: IgG
• 輸送温度: ambient
• 保管温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

Mouse monoclonal antibody lyophilized from cell culture supernatant. Recognizes the ~115 kDa subunit of the NMDAR (N-methyl-D-aspartate receptor) protein.

Recognizes the ~115 kDa NMDA receptor NR1 subunit in rat cortex tissue extracts.

This Anti-Pan-NMDA NR1 Mouse mAb (R1JHL) is validated for use in Immunoblotting for the detection of Pan-NMDA NR1.

免疫原

Rat

a recombinant fusion protein consisting of amino acids 1-564 of rat NMDA NR1

アプリケーション

Immunoblotting (1:300, colorimetric)

Immunoprecipitation (3 µg/200 µg lysate)

警告

Toxicity: Standard Handling (A)

物理的形状

Lyophilized from cell culture supernatant.

再構成

Reconstitute in 50 µl PBS (137 mM NaCl, 7.5 mM Na₂HPO₄, 2.7 mM KCl, 1.5 mM KH₂P0₄, pH 7.4)Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.

アナリシスノート

Positive Control
Rat cortex tissue extracts

その他情報

Does not cross-react with other NMDAR subunits. For immunoblotting, dilute in TBST containing 5% nonfat milk and 0.05% sodium azide. Antibody may be used up to 5 times in immunoblotting experiments without significant loss in signal strength. Store diluted antibody at 4°C between uses. Variables associated with assay conditions will dictate the proper working dilution.

Laurie, D.J., and Seeburg, P.H. 1994. J. Neurosci.14, 3180
Ishii, T., et al. 1993. J. Biol. Chem.268, 2836.
Monyer, H., et al. 1992. Science256, 1217.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable