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Key Documents

安全性情報

393206

Sigma-Aldrich

Anti-HNE Fluorophore Rabbit pAb

liquid, Calbiochem®

別名:

Anti-N α-Acetyllysine-4-hydroxy-2-nonenal Fluorophore, Anti-Nα-Acetyllysine-4-hydroxy-2-nonenal Fluorophore

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About This Item

UNSPSCコード:
12352203
NACRES:
NA.43

由来生物

rabbit

品質水準

抗体製品の状態

diluted serum

抗体製品タイプ

primary antibodies

クローン

polyclonal

形状

liquid

化学種の反応性(ホモロジーによる予測)

all

メーカー/製品名

Calbiochem®

保管条件

OK to freeze
avoid repeated freeze/thaw cycles

アイソタイプ

IgG

輸送温度

wet ice

保管温度

−70°C

ターゲットの翻訳後修飾

unmodified

詳細

Anti-HNE Fluorophore, rabbit polyclonal, recognizes the HNE-derived chromphohore of the 2:1 lysine-HNE-fluorophore. It is validated for use in ELISA, Western blotting, and immunohistochemistry.
Rabbit polyclonal antibody supplied as diluted serum. Recognizes the HNE-derived chromophore of the 2:1 lysine-HNE fluorophore.
Recognizes the HNE-derived chromphohore of the 2:1 lysine-HNE-fluorophore.

免疫原

a 2:1 Nα-acetyllysine-HNE fluorophore, conjugated to KLH

アプリケーション

ELISA (1:500-1:1000)

Immunoblotting (1:1000-1:5000)

Immunohistochemistry (1:100-1:500)

警告

Toxicity: Standard Handling (A)

物理的形状

Serum diluted 1:1 with glycerol.

再構成

Following initial thaw, aliquot and freeze (-70°C).

その他情報

Due to the small epitope size, overnight incubation with the antibody greatly increases sensitivity by immunoblotting. Determined by competitive ELISA and immunoblotting to recognize the chromophore structure of fluorophore derived from the reaction of 4-HNE with primary amines. Does not recognize 1:1 cysteine-, 1:1 histidine-, or 1:1 lysine-HNE Michael Adducts. Variables associated with assay conditions will dictate the proper working dilution.
Tsai, L., et al. 1998. Proc. Natl. Acad. Sci. USA95, 7975.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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保管分類コード

10 - Combustible liquids

WGK

WGK 1


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

393206-100UL:
393206-UL:


試験成績書(COA)

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文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Ernst-Bernhard Kayser et al.
PloS one, 11(1), e0148219-e0148219 (2016-01-30)
Lack of NDUFS4, a subunit of mitochondrial complex I (NADH:ubiquinone oxidoreductase), causes Leigh syndrome (LS), a progressive encephalomyopathy. Knocking out Ndufs4, either systemically or in brain only, elicits LS in mice. In patients as well as in KO mice distinct
Erik P Rader et al.
Rejuvenation research, 20(2), 93-102 (2016-07-06)
Exercise is the most accessible, efficacious, and multifactorial intervention to improve health and treat chronic disease. High-intensity resistance exercise, in particular, also maximizes skeletal muscle size and strength-outcomes crucial at advanced age. However, such training is capable of inducing muscle
Marshall A Naimo et al.
Journal of applied physiology (Bethesda, Md. : 1985), 126(4), 1074-1087 (2019-01-25)
The purpose of this study was to characterize the growth and remodeling molecular signaling response in aged skeletal muscle following 1 mo of "resistance-type exercise" training. Male Fischer 344 × Brown Norway hybrid rats aged 3 (young) and 30 mo
Kaitlin N Allen et al.
The Journal of experimental biology, 222(Pt 12) (2019-06-07)
Seals experience repeated bouts of ischemia-reperfusion while diving, potentially exposing their tissues to increased oxidant generation and thus oxidative damage and accelerated aging. We contrasted markers of oxidative damage with antioxidant profiles across age and sex for propulsive (longissismus dorsi)
John M Lawler et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 306(7), R470-R482 (2014-01-31)
Reduced mechanical loading during bedrest, spaceflight, and casting, causes rapid morphological changes in skeletal muscle: fiber atrophy and reduction of slow-twitch fibers. An emerging signaling event in response to unloading is the translocation of neuronal nitric oxide synthase (nNOSμ) from

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