2384

Creatine Phosphokinase, Rabbit Skeletal Muscle

• 別名: Creatine Phosphokinase, Rabbit Skeletal Muscle, rCPK
• CAS番号: 9001-15-4
• Enzyme Commission number: 2.7.3.2 (BRENDA, IUBMB)
• MDL番号: MFCD00130876
• UNSPSCコード: 12352202
• NACRES: NA.21

特性

• 由来生物: rabbit skeletal muscle
• 品質水準: 200
• 形状: lyophilized
• 比活性: ≥300 U/mg, 25 °C; 750 U/mg, 37 °C
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; desiccated (hygroscopic)
• 溶解性: 0.1 M imidazole buffer, pH 6.6: soluble; 0.5% NaHCO₃, pH 9.0: soluble
• その他の活性: ATPase, hexokinase, myokinase <0.001%
• 輸送温度: ambient
• 保管温度: −20°C

詳細

詳細

Native creatine phosphokinase from rabbit muscle. Catalyzes the conversion of creatine and ATP to creatine phosphate and ADP. Has an optimal pH of 9.0 for the forward reaction and 6.0-7.0 for the reverse reaction.

Native creatine phosphokinase from rabbit muscle. Catalyzes the reversible conversion of creatine and ATP to creatine phosphate and ADP. Has an optimal pH of 9.0 for the forward reaction and 6.0-7.0 for the reverse reaction.

警告

Toxicity: Standard Handling (A)

単位の定義

One unit is defined as the amount of enzyme that will catalyze the conversion of 1.0 µmol creatine phosphate per min at 25°C or 37°C, pH 6.8.

再構成

Following reconstitution in 0.5% NaHCO₃, refrigerate (4°C). Do not freeze or shake, as SH groups may be oxidized and form linkages between protein molecules, resulting in precipitation, turbidity, and loss of activity. NaHCO₃ stock solutions are stable for 2-3 days at 4°C. Following reconstitution in 0.1 M imidazole buffer, aliquot and freeze (-20°C). Imidazole stock solutions are stable for up to 4 weeks at -20°C. Avoid freeze/thaw cycles, as 20-30% of activity will be lost with each freeze/thaw cycle.

その他情報

Bessman, S.P. 1987. Anal. Biochem.161, 519.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable