219402

Cathepsin L, Human Liver

Cathepsin L, Human Liver, CAS 60616-82-2, is a native, the most potent of all the lysosomal proteinases. Plays a major role in the proteolysis of both cellular and endocytosed macromolecules.

• 別名: CTSL, CTSL1, Human Cathepsin L, Major excreted protein (MEP)
• Enzyme Commission number: 3.4.22.15 (BRENDA, IUBMB)
• UNSPSCコード: 12352202
• NACRES: NA.77

特性

• 由来生物: human liver
• 品質水準: 100
• 形状: liquid
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; avoid repeated freeze/thaw cycles
• テクニック: activity assay: suitable
• 適合性: suitable for molecular biology
• アプリケーション: life science and biopharma
• 輸送温度: wet ice
• 保管温度: −70°C
• 遺伝子情報: human ... CTSL(1514)

詳細

詳細

Research area: Cell Signaling
Cathepsin L, Human Liver, native, is the most potent of all the lysosomal proteinases. Cathepsin L (CTSL) belongs to the papain subfamily of cysteine proteases and is mainly located in endolysosomal vesicles.

生物化学的／生理学的作用

Cathepsin L (CTSL) has higher activity than cathepsins B and H in the degradation of a variety of physiological protein substrates. It is believed to be responsible for the generation of endostatin from the NC1 domain in collagen XVII. It is responsible for regulating cell cycle, nucleotide-binding domain, leucine-rich–containing family, pyrin domain–containing-3 (NLRP3) inflammasome activation, proteolytic processing of Bid during apoptosis, and TGF-β signaling. It can degrade a wide range of proteins, encompassing enzymes, receptors, and transcription factors. Additionally, it produces active enzymes, receptors, and biologically active peptides through controlled proteolysis. Moreover, Cathepsin L has been observed to enhance tumor cell migration by lowering cell-cell adhesion and breaking down elements of the extracellular matrix. The expression of CTSL escalates in several types of cancers, including glioma, melanoma, pancreatic, prostate, and breast cancer. It plays a major role in the proteolysis of both cellular and endocytosed macromolecules.

包装

Please refer to vial label for lot-specific concentration.

警告

Toxicity: Standard Handling (A)

単位の定義

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of Z-FR-AFC per min at 25°C, pH 5.5.

物理的形状

In 400 mM NaCl, 20 mM malonate buffer, 1 mM EDTA, pH 5.5.

調製ノート

Prepared from tissue of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

再構成

Following initial thaw, aliquot and freeze (-70°C).

その他情報

Note: 1 mU = 1 milliunit

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2