197217

Bcl-x_L BH4₄₋₂₃, Human, Cell-Permeable

A cell-permeable peptide that prevents apoptotic cell death by directly binding to the voltage-dependent anion channel (VDAC) and blocking its activity.

• 別名: Bcl-x_L BH4₄₋₂₃, Human, Cell-Permeable, HIV-TAT₄₈₋₅₇-β-Ala-Bcl-x _L BH4₄₋₂₃, Ac-GRKKRRQRRR-βA-SNRELVVDFLSYKLSQKGYS-OH
• 実験式（ヒル表記法）: C₁₆₅H₂₇₉N₅₉O₄₆
• 分子量: 3825.35
• UNSPSCコード: 12352202
• NACRES: NA.77

特性

• 品質水準: 100
• アッセイ: ≥95% (HPLC)
• フォーム: lyophilized
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; desiccated (hygroscopic)
• 溶解性: 20% acetic acid: 5 mg/mL; DMSO: 5 mg/mL
• 輸送温度: ambient
• 保管温度: −20°C

詳細

詳細

A cell-permeable peptide that prevents apoptotic cell death by directly binding to the voltage-dependent anion channel (VDAC) and blocking its activity. Leads to the inhibition of cytochrome c release and loss of mitochondrial membrane potential (ΔΨ_m). Contains the conserved N-terminal homology domain (BH4) of Bcl-x_L (amino acids 4 - 23) that has been shown to be essential for inhibiting VDAC activity in liposomes and in isolated mitochondria. The BH4 domain is linked to a carrier peptide, a 10-amino acid HIV-TAT_48-57 sequence with a β-alanine residue as a spacer for maximum flexibility. Following its uptake, it is mainly localized to the mitochondria.

A highly cell-permeable peptide that prevents apoptotic cell death by directly binding to the voltage-dependent anion channel (VDAC) and blocking its activity. Ultimately results in the inhibition of cytochrome c release and loss of mitochondrial membrane potential (ΔΨm). Contains the conserved N-terminal homology domain (BH4) of Bcl-x_L (amino acids 4-23) that has been shown to be essential for inhibiting VDAC activity in liposomes and isolated mitochondria. The BH4 domain is linked to the carrier peptide, the 10-amino acid HIV-TAT_48-57 sequence with a β-alanine residue as a spacer for maximum flexibility. Following its uptake, it is mainly localized in the mitochondria.

生物化学的／生理学的作用

Cell permeable: yes

Primary Target
Voltage-dependent anion channel (VDAC)

Product does not compete with ATP.

Reversible: yes

包装

Packaged under inert gas

警告

Toxicity: Standard Handling (A)

シーケンス

Ac-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-βAla-Ser-Asn-Arg-Glu-Leu-Val-Val-Asp-Phe-Leu-Ser-Tyr-Lys-Ser-Gln-Lys-Gly-Tyr-Ser-OH

物理的形状

Supplied as a trifluoroacetate salt.

再構成

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

その他情報

Chen m., et al. 2002. J. Biol. Chem. 277, 29181.
Futaki, S., et al. 2001. J. Biol. Chem.276, 5836.
Shimizu, S., et al. 2000. Proc. Natl. Acad. Sci. USA97, 3100.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable