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詳細
All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
The ChIPAb+ Trimethyl-Histone H3 (Lys4) set includes the Anti-trimethyl-Histone H3 (Lys4) antibody, a negative control antibody (purified Mouse IgG), and qPCR primers which amplify a 166 bp region within the promoter of the human GAPDH gene. The trimethyl-Histone H3 (Lys4) and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of trimethyl-histone H3 (Lys4)-associated chromatin.
The ChIPAb+ Trimethyl-Histone H3 (Lys4) set includes the Anti-trimethyl-Histone H3 (Lys4) antibody, a negative control antibody (purified Mouse IgG), and qPCR primers which amplify a 166 bp region within the promoter of the human GAPDH gene. The trimethyl-Histone H3 (Lys4) and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of trimethyl-histone H3 (Lys4)-associated chromatin.
The previously assigned protein identifier Q66I33 has been merged into P84243. Full details can be found on the UniProt database.
特異性
Recognizes histone H3, Mr 17 kDa, trimethylated at Lys4.
The immunogen sequence is identical in a wide range of animal and plant species.
免疫原
Epitope: a.a. 1-12
The trimethyl-histone H3 (Lys4) purified antibody is made against a synthetic peptide (trimethylated at Lys4) corresponding to amino acids 1-12 of human histone H3.
アプリケーション
Research Category
エピジェネティクス及び核内機能分子
エピジェネティクス及び核内機能分子
Research Sub Category
エピジェネティクス
エピジェネティクス
Chromatin Immunoprecipitation:
Sonicated chromatin prepared from untreated or colcemid treated HeLa cells (1 X 106 cell equivalents per IP) was subjected to chromatin immunoprecipitation using 2 µg of either a normal mouse IgG or Anti-trimethyl-Histone H3 (Lys4) antibody and the Magna ChIP G Kit (Cat. #17-611). Successful immunoprecipitation of trimethyl-Histone H3 (Lys4) associated DNA fragments was verified by qPCR using primers ampliflying a region of the human B-Globin promoter or using GAPDH promoter Control Primers (Please see figures). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
Please refer to the EZ-Magna G ChIP (Cat. #17-408) or EZ-ChIP (Cat. #17-371) protocol for experimental details.
Western Blot Analysis:
Representative lot data.
HeLa acid extract were resolved by electrophoresis, transferred to PVDF membrane and probed with Anti-trimethyl Histone H3 (Lys4), clone CMA304 at 1 μg/ml (lane 1) or 0.5 μg/ml (lane 2). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP (Cat. #AP124P) and a chemiluminescence detection system (Please see figures).
Sonicated chromatin prepared from untreated or colcemid treated HeLa cells (1 X 106 cell equivalents per IP) was subjected to chromatin immunoprecipitation using 2 µg of either a normal mouse IgG or Anti-trimethyl-Histone H3 (Lys4) antibody and the Magna ChIP G Kit (Cat. #17-611). Successful immunoprecipitation of trimethyl-Histone H3 (Lys4) associated DNA fragments was verified by qPCR using primers ampliflying a region of the human B-Globin promoter or using GAPDH promoter Control Primers (Please see figures). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
Please refer to the EZ-Magna G ChIP (Cat. #17-408) or EZ-ChIP (Cat. #17-371) protocol for experimental details.
Western Blot Analysis:
Representative lot data.
HeLa acid extract were resolved by electrophoresis, transferred to PVDF membrane and probed with Anti-trimethyl Histone H3 (Lys4), clone CMA304 at 1 μg/ml (lane 1) or 0.5 μg/ml (lane 2). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP (Cat. #AP124P) and a chemiluminescence detection system (Please see figures).
This ChIPAb+ Trimethyl-Histone H3 (Lys4) -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
包装
25 assays per kit, ~2μg per chromatin immunoprecipitation
品質
Chromatin Immunoprecipitation:
Sonicated chromatin prepared from colcemid-reated HeLa cells (1 X 106 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µg of either normal mouse IgG or Anti-trimethyl-Histone H3 (Lys4) antibody and the Magna ChIP G Kit (Cat. #17-611). Successful immuno-precipitation of trimethyl-histone H3 (Lys4) associated DNA fragments was verified by qPCR using Control Primers (Please see figures).
Please refer to the EZ-Magna G ChIP (Cat. #17-409) or EZ-ChIP (Cat. #17-371) protocol for experimental details.
Sonicated chromatin prepared from colcemid-reated HeLa cells (1 X 106 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µg of either normal mouse IgG or Anti-trimethyl-Histone H3 (Lys4) antibody and the Magna ChIP G Kit (Cat. #17-611). Successful immuno-precipitation of trimethyl-histone H3 (Lys4) associated DNA fragments was verified by qPCR using Control Primers (Please see figures).
Please refer to the EZ-Magna G ChIP (Cat. #17-409) or EZ-ChIP (Cat. #17-371) protocol for experimental details.
ターゲットの説明
Trimethyl histone H3 at ~17 kDa
物理的形状
Anti-trimethyl-Histone H3 (Lys4) (mouse monoclonal IgG1, Clone CMA304). One vial containing 50 μg of protein G purified antibody in 50 μL PBS containing 0.05% sodium. Store at -20°C.
Normal Mouse IgG. Two vials containing 25 μg purified mouse IgG in 25 μL storage buffer containing 0.1% sodium azide. Store at -20°C.
Control Primers. One vial containing 75 μL of 5 μM of each primer specific for the promoter region of GAPDH. Store at -20°C.
FOR: TAC TAG CGG TTT TAC GGG CG
REV: TCG AAC AGG AGG AGC AGA GAG CGA
Normal Mouse IgG. Two vials containing 25 μg purified mouse IgG in 25 μL storage buffer containing 0.1% sodium azide. Store at -20°C.
Control Primers. One vial containing 75 μL of 5 μM of each primer specific for the promoter region of GAPDH. Store at -20°C.
FOR: TAC TAG CGG TTT TAC GGG CG
REV: TCG AAC AGG AGG AGC AGA GAG CGA
保管および安定性
Stable for 1 year at -20°C from date of receipt. Aliquot upon thawing, avoid freeze thaw cycles.
アナリシスノート
Control
Included negative control mouse IgG antibody and control primers specific for human GAPDH promoter.
Included negative control mouse IgG antibody and control primers specific for human GAPDH promoter.
法的情報
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
保管分類コード
10 - Combustible liquids
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
毒物及び劇物取締法
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PRTR
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消防法
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労働安全衛生法名称等を表示すべき危険物及び有害物
キットコンポーネントの情報を参照してください
労働安全衛生法名称等を通知すべき危険物及び有害物
キットコンポーネントの情報を参照してください
カルタヘナ法
キットコンポーネントの情報を参照してください
Jan Code
キットコンポーネントの情報を参照してください
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
The gene signature in CCAAT-enhancer-binding protein ? dysfunctional acute myeloid leukemia predicts responsiveness to histone deacetylase inhibitors.
Liss, A; Ooi, CH; Zjablovskaja, P; Benoukraf, T; Radomska, HS; Ju, C; Wu, M; Balastik et al.
Haematologica null
Jonathan C Irish et al.
Molecular oncology, 10(6), 850-865 (2016-03-24)
The 8p11-p12 amplicon occurs in approximately 15% of breast cancers in aggressive luminal B-type tumors. Previously, we identified WHSC1L1 as a driving oncogene from this region. Here, we demonstrate that over-expression of WHSC1L1 is linked to over-expression of ERα in
Judy A Brusslan et al.
Plant physiology, 168(4), 1246-1261 (2015-03-25)
The genome-wide abundance of two histone modifications, H3K4me3 and H3K9ac (both associated with actively expressed genes), was monitored in Arabidopsis (Arabidopsis thaliana) leaves at different time points during developmental senescence along with expression in the form of RNA sequencing data.
Judy A Brusslan et al.
PloS one, 7(3), e33151-e33151 (2012-03-20)
Leaf senescence is the orderly dismantling of older tissue that allows recycling of nutrients to developing portions of the plant and is accompanied by major changes in gene expression. Histone modifications correlate to levels of gene expression, and this study
Liang Yan et al.
Molecular pharmacology, 92(2), 113-123 (2017-05-27)
CYP3A4 is one of the major drug-metabolizing enzymes in human and is responsible for the metabolism of 60% of clinically used drugs. Many drugs are able to induce the expression of CYP3A4, which usually causes drug-drug interactions and adverse drug
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