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由来生物
human
品質水準
リコンビナント
expressed in baculovirus infected Sf21 cells
分子量
Mw 67.3 kDa
メーカー/製品名
Upstate®
テクニック
activity assay: suitable (kinase)
NCBIアクセッション番号
UniProtアクセッション番号
詳細
N-terminal GST-tagged recombinant human B-Raf residues 416-end, containing a V599E mutation (also designated V600E)
Product Source: Expressed by baculovirus in Sf21 insect cells
アプリケーション
Research Category
炎症及び免疫
炎症及び免疫
生物化学的/生理学的作用
Protein Target: B-Raf
Target Sub-Family: TKL
包装
Also available in 250 µg size --call for pricing and availability and reference catalog number 14-557M when ordering the 250 µg size.
品質
routinely evaluated by phosphorylation of MBP substrate in vitro
物理的形状
Glutathione agarose affinity chromatography
保管および安定性
6 months at -70°C
その他情報
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
法的情報
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
シグナルワード
Warning
危険有害性情報
危険有害性の分類
Skin Sens. 1
保管分類コード
10 - Combustible liquids
WGK
WGK 2
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
毒物及び劇物取締法
劇物
Jan Code
14-557M:
14-557:
試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Vanessa C Gray-Schopfer et al.
Cancer metastasis reviews, 24(1), 165-183 (2005-03-24)
Melanoma is a form of skin cancer that has a poor prognosis and which is on the rise in Western populations. If detected early, it is easily treated by surgical excision. However, once melanoma metastasises it is notoriously resistant to
Mathew J Garnett et al.
Cancer cell, 6(4), 313-319 (2004-10-19)
RAF proteins are components of a conserved signaling pathway that regulates cellular responses to extracellular signals. Despite over 20 years of research into the regulation and function of the RAF proteins, it was only realized recently that the B-RAF isoform
Stevan R Hubbard
Cell, 116(6), 764-766 (2004-03-24)
A study by Wan et al. in this issue of Cell demonstrates that the majority of oncogenic mutations in the B-Raf protein kinase result in increased catalytic activity, through disruption of the autoinhibited state of the kinase domain. Surprisingly, several
Kathryn E Mercer et al.
Biochimica et biophysica acta, 1653(1), 25-40 (2003-06-05)
A recent report has shown that activating mutations in the BRAF gene are present in a large percentage of human malignant melanomas and in a proportion of colon cancers. The vast majority of these mutations represent a single nucleotide change
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