1.25062

Fractogel^® EMD COO^- (M)

MiniChrom Fractogel^® COO (M), volume 1 mL, prepacked column

• 別名: MiniChrom Column Fractogel^® COO (M), 1 ml
• UNSPSCコード: 41115711

特性

• ligand: (Carboxyethyl )
• 物質: polypropylene housing
• 製品種目: Fractogel^®
• 形状: prepacked column
• パラメーター: ≤8 bar pressure; ~300 cm/hr max. flow rate
• column I.D. × L: 8 mm × 20 mm
• 容量: 1 mL
• マトリックス活性基: Methacrylate
• 平均粒径: 40-90 μm
• キャパシティ: ~100 mL, resin binding capacity
• アプリケーション: mAb; recombinant protein; vaccine development
• 分離法: cation exchange
• 保管温度: 15-25°C

詳細

詳細

8 x 20mm

特徴および利点

MiniChrom Column Fractogel^® COO (M), 1 ml enables:

• Good resolution between monomers and aggregates
• Efficient aggregate and HCP removal combined with high yield
• Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution

Due to the titration behavior, the ion exchange capacity can be used from pH 6 to pH 12. The separation of proteins is based on reversible electrostatic interactions between the negatively charged regions of the proteins′ surface and the support. Proteins are retained efficiently on Fractogel^® EMD COO^- (M) when the pH of the buffer is about 1 unit below their isoelectric points (pl).

The strength of the binding depends on the following:

• the buffer system
• pH value of the buffer which determines the surface charge of the protein
• the degree of the ionization of the functional groups of the exchanger
• the concentration of the counter ions
• the charge density on the support (protein binding capacity)

アナリシスノート

Appearance: passes test

法的情報

FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany