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Merck

114666

Sigma-Aldrich

Actinomycin D, Streptomyces sp.

Anti-neoplastic antibiotic. Inhibits DNA-primed RNA polymerase by complexing with DNA via deoxyguanosine residues.

別名:

Actinomycin D, Streptomyces sp., Dactinomycin, RNA Polymerase I Inhibitor I, Pol I Inhibitor I

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About This Item

実験式(ヒル表記法):
C62H86N12O16
CAS番号:
分子量:
1255.42
MDL番号:
UNSPSCコード:
12352200

品質水準

アッセイ

≥98% (HPLC)

形状

crystalline solid

メーカー/製品名

Calbiochem®

保管条件

OK to freeze
desiccated (hygroscopic)
protect from light

red

溶解性

DMSO: 1 mg/mL
chloroform: soluble
methanol: soluble

輸送温度

ambient

保管温度

2-8°C

InChI

1S/C62H86N12O16/c1-27(2)42-59(84)73-23-17-19-36(73)57(82)69(13)25-38(75)71(15)48(29(5)6)61(86)88-33(11)44(55(80)65-42)67-53(78)35-22-21-31(9)51-46(35)64-47-40(41(63)50(77)32(10)52(47)90-51)54(79)68-45-34(12)89-62(87)49(30(7)8)72(16)39(76)26-70(14)58(83)37-20-18-24-74(37)60(85)43(28(3)4)66-56(45)81/h21-22,27-30,33-34,36-37,42-45,48-49H,17-20,23-26,63H2,1-16H3,(H,65,80)(H,66,81)(H,67,78)(H,68,79)

InChI Key

RJURFGZVJUQBHK-UHFFFAOYSA-N

詳細

Anti-neoplastic antibiotic. Inhibits DNA-primed RNA polymerase by complexing with DNA via deoxyguanosine residues. At higher concentrations, DNA polymerase is inhibited. Also acts as a competitive inhibitor of serine proteases. Inhibits cell growth and colony formation in synchronized HeLa cells. Induces apoptosis in many cell lines. However, actinomycin D has also been shown to suppress etoposide-induced apoptosis in PC12 cells. Note: 1 set = 20 x 200 µg.
Antineoplastic antibiotic. Inhibits DNA-primed RNA polymerase by complexing with DNA via deoxyguanosine residues. A DNA-intercalator with growth inhibitory activity against certain leukemias and sarcomas. At higher concentrations, DNA polymerase is inhibited. Inhibits cell growth and colony formation in synchronized HeLa cells. A potent inducer of apoptosis in many cell lines; however, actinomycin D has also been shown to suppress programmed cell death of PC12 cells induced by etoposide, an inhibitor of topoisomerase II. Acts as a competitive inhibitor of serine protease from microorganisms. The growth of S. aureus is inhibited by 0.25-0.75 µg/ml, whereas a concentration of 100 µg/ml is required to inhibit E. coli due to permeability differences. Heat resistant and stable between pH 2 and 11; decomposed by strong acids and bases. λmax 441 nm ±2 nm. Note: 1 set = 20 vials x 200 µg.
Stability: heat resistant and stable between pH 2 and 11. Decomposed by strong acids and bases.

生物化学的/生理学的作用

Primary Target
serine proteases
Secondary Target
cell growth and colony formation in synchronized HeLa cells

警告

Toxicity: Highly Toxic & Carcinogenic / Teratogenic (I)

再構成

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 3 months at -20°C.

その他情報

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Nakajima, M., et al. 1994. Neurosci. Lett.176, 161.
Wu, M.H., and Yung, B.Y. 1994. Eur. J. Pharmacol. 270, 203.
Betzel, C., et al. 1993. Biochim. Biophys. Acta 1161, 47.
Yung, B.Y., et al. 1992. Int. J. Cancer52, 317.
Martin, S.J., et al. 1990. J. Immunol.145, 1859.
Yung, B.Y., et al. 1990. Cancer Res.50, 5987.
White, R.J., and Phillips, D.R. 1985. Biochemistry27, 9122.
Madharavao, M., et al. 1978. J. Med. Chem.21, 958.
Sengupta, S.K., et al. 1975. J. Med. Chem.18, 1175.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

ピクトグラム

Skull and crossbonesHealth hazard

シグナルワード

Danger

危険有害性情報

危険有害性の分類

Acute Tox. 2 Oral - Carc. 1B - Repr. 1B

保管分類コード

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

114666-1SET:
114666-5MG:
114666-0GM:


試験成績書(COA)

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文書ライブラリにアクセスする

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Cell-to-cell heterogeneity is a feature of the tumor necrosis factor (TNF)-stimulated inflammatory response mediated by the transcription factor NF-κB, motivating an exploration of the underlying sources of this noise. Here, we combined single-transcript measurements with computational models to study transcriptional
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Cell reports, 30(11), 3605-3615 (2020-03-19)
Multiple cancer-related genes both promote and paradoxically suppress growth initiation, depending on the cell context. We discover an explanation for how this occurs for one such protein, Stat3, based on asymmetric cell division. Here, we show that Stat3, by Stathmin/PLK-1
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Biology of the cell, 106(10), 359-376 (2014-07-25)
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