1.10765

EC (Escherichia coli) broth

GranuCult^® prime, suitable for microbiology, for Escherichia coli

• 別名: E. coli selective medium, Escherichia coli broth, EC broth
• UNSPSCコード: 41106213
• NACRES: NA.74

特性

• 認証: FDA-BAM; ISO 7251
• 品質水準: 200
• 無菌性: non-sterile
• 形状: granular
• 包装: pkg of 500 g
• メーカー／製品名: GranuCult^® prime
• 保管条件: dry media (Tightly closed.)
• テクニック: microbiological culture: suitable
• 色: beige
• pH: 6.7-7.1 (25 °C, 37 g/L in H₂O, after autoclaving)
• 溶解性: 74 g/L
• アプリケーション: food and beverages; microbiology
• 保管温度: 15-25°C
• 適合性: Escherichia coli; Escherichia spp.

詳細

詳細

Escherichia coli broth is suitable for the selective enrichment, enumeration, and confirmation of coliform bacteria. The lactose content of this medium favors the growth of lactose-positive bacteria, especially coliform bacteria, and E. coli. The bile salts inhibit the growth of Gram-positive bacteria or microorganisms that are not adapted to the intestinal environment. Lactose-positive bacteria metabolize lactose with gas formation.

アプリケーション

Escherichia coli broth is suitable for the selective enrichment, enumeration, and confirmation of coliform bacteria from food and animal feed, and other materials.

特徴および利点

• GranuCult^® prime offers superior granulated culture media
• Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment
• Excellent wettability, solubility, and free-flowing properties
• Convenient, with minimal component separation and clumping, even under warm or humid conditions
• High batch-to-batch reproducibility
• Prolonged shelf life of up to five years
• High number of test strains exceeding all regulatory demands
• Granulation technology allows many supplements to be included, with no need to add these separately

アナリシスノート

Appearance (clearness): clear
Appearance (color): yellowish to yellowish-brown
pH-value (25 °C): 6.6 - 7.0
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≤ 1000
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)): ≤ 1000
Inoculum on reference medium (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): ≥ 1E+04
Inoculum on reference medium (Enterococcus faecalis ATCC 19433 (WDCM 00009)): ≥ 1E+04
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): +
Growth (Escherichia coli ATCC 8739 (WDCM 00012)): +
Growth (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): no limit
Growth (Enterococcus faecalis ATCC 19433 (WDCM 00009)): total inhibition
Gas (Escherichia coli ATCC 25922 (WDCM 00013)): +
Gas (Escherichia coli ATCC 8739 (WDCM 00012)): +
Gas (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): -
Gas (Enterococcus faecalis ATCC 19433 (WDCM 00009)): -
Escherichia coli: 24 ± 2 hours at 44 ± 1 °C
other teststrains: 48 ± 2 hours at 44 ± 1 °C

脚注

We offer two media types: the superior granulated GranuCult^® prime and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

法的情報

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 2