1.04187

Amido black 10 B (C.I.20470)

• 実験式（ヒル表記法）: C₂₂H₁₄N₆Na₂O₉S₂
• CAS番号: 1064-48-8
• 分子量: 616.49
• MDL番号: MFCD00004017
• NACRES: NA.41

特性

• フォーム: solid
• 品質水準: 200
• 有効性: >5000 mg/kg LD₅₀, oral (Rat)
• 組成: Dye, ≥80% spectrophotometric assay
• pH: 9 (25 °C, 10 g/L in H₂O)
• 溶解性: 10 g/L
• かさ密度: 670 kg/m³
• アプリケーション: diagnostic assay manufacturing; hematology; histology
• 保管温度: 2-30°C
• InChI: 1S/C22H16N6O9S2.2Na/c23-19-18-12(11-17(39(35,36)37)21(22(18)29)27-24-13-4-2-1-3-5-13)10-16(38(32,33)34)20(19)26-25-14-6-8-15(9-7-14)28(30)31;;/h1-11,24H,23H2,(H,32,33,34)(H,35,36,37);;/q;2*+1/p-2/b26-25?,27-21+;;
• InChI Key: HKBVRFLHNUEVRO-DWTBGCDMSA-L

詳細

詳細

Amido black 10 B (C.I.20470) for electrophoresis, is used for staining connective tissue, in electrophoresis, and as a redox indicator. Proteins possess free amino and carboxyl groups and are charged positively or negatively in aqueous solutions, depending on the pH. In an alkaline milieu they are negatively charged and migrate in the electric field towards the anode at a speed that depends on the number of charges they carry. This difference in migration velocities makes it possible to separate the serum protein mixture into groups of individual components. The separated protein fractions are stained with amido black and measured photometrically or densitometrically. As a rule, five fractions are observed after staining - albumin, alpha 1- and alpha 2-globulins, beta 1- and beta 2-globulins, and beta-globulins - where albumin has the highest migration velocity and gamma-globulin the lowest. Differentiation of serum proteins by electrophoresis is important for the diagnosis and prognosis of numerous diseases. The dye has a constant dye content and a reliable high quality, which is one of the major precondition for a reproducible diagnostic staining.

アプリケーション

for electrophoresis

アナリシスノート

Dye content (spectrophotometrically): ≥ 80 %
Identity
- sodium:
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ max (water): 617 - 620 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.01 g/l; water): 750 - 960
TLC-Test: passes test
Loss on drying (110 °C): ≤ 10 %
Suitability for electrophoresis: passes test
Suitability as redox indicator: passes test

安全性情報

• ピクトグラム: GHS07
• シグナルワード: Warning
• 危険有害性情報: H317
• 注意書き: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• 危険有害性の分類: Skin Sens. 1B
• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable