1.00215

GranuCult^® prime Hektoen Enteric Agar

according to ISO 21567, FDA-BAM, according to ISO 6579, selective and differential for Salmonella spp., selective and differential for Shigella spp., suitable for microbiology

• 別名: HE Agar, HEA, Hektoen Enteric Agar
• UNSPSCコード: 41106200
• NACRES: NA.74

特性

• 認証: FDA-BAM; according to ISO 21567; according to ISO 6579
• 品質水準: 200
• 無菌性: non-sterile
• 滅菌適合性: autoclavable compatible
• 形状: granular
• 包装: pkg of 500 g
• メーカー／製品名: GranuCult^® prime
• 保管条件: protect from light (Keep dry and tightly closed)
• テクニック: microbiological culture: suitable
• 色: dark green
• pH: 7.5-7.9 (25 °C, 75 g/L in H₂O)
• 溶解性: 75 g/L
• アプリケーション: clinical; food and beverages; industrial qc; pharmaceutical; microbiology
• 保管温度: 15-25°C
• 適合性: selective and differential for Salmonella spp.; selective and differential for Shigella spp.

詳細

詳細

GranuCult^® prime Hektoen Enteric Agar provides good colonial differentiation and inhibits some coliforms and other non-lactose-fermenting bacteria, thereby facilitating the isolation and differentiation of Salmonella spp. and Shigella spp. from samples such as food chain and other materials.Lactose-positive colonies have a different color from lactose-negative ones due to the presence of the two indicators for carbohydrate dissimilation: bromothymol blue and acidic fuchsin. The two additional carbohydrates, sucrose (saccharose) and salicin, also cause the color difference in colonies. The black color in the H₂S-positive colonies is due to thiosulfate as a reactive compound with an iron salt as an indicator.This culture medium is moderately to highly selective. The mixture of bile salts suppresses the growth of most of the accompanying microorganisms. The inhibitory effect of bile salts on the growth of Shigella spp. is reduced by relatively large amounts of peptone and carbohydrates, which allow good growth. Agar is the solidifying agent.

アプリケーション

GranuCult^® prime Hektoen Enteric Agar is suitable for the isolation and differentiation ofSalmonella and Shigella from the food chain and other materials.

特徴および利点

• GranuCult^® offers superior granulated culture media.
• Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment.
• Excellent wettability, solubility, and free-flowing properties
• Convenient, with minimal component separation and clumping, even under warm or humid conditions.
• High batch-to-batch reproducibility
• Prolonged shelf life of up to five years
• High number of test strains exceeding all regulatory demands
• Granulation technology allows many supplements to be included, with no need to add these separately

保管および安定性

Self-prepared plates can be stored in the dark and protected against evaporation:

• acc. to ISO 21567 at (5 ± 3 °C) for up to 2 weeks
• acc. to FDA-BAM at (4 ± 2 °C) for up to 30 days
• acc to Corry et al., (2012) at (5 ± 3 °C) for up to 3 weeks

アナリシスノート

• Salmonella spp. produce transparent green to blue-green colonies, with or without black centers, or may appear completely black due to H₂S production.
• Shigella spp. produce green, transparent moist colonies.
• Lactose, Sucrose or Salicin fermenting gram-negative bacteria may be inhibited or produce red to salmon colored colonies with or without precipitation zone.

その他情報

• Do not use clumped or discolored medium.
• The prepared medium is clear to slightly opalescent and blue-green to blue-grey.
• Before inoculation, allow the prepared medium to equilibrate at room temperature if it is stored at a lower temperature.
• There should be no visible moisture on the plates before use. When moisture is present, the plates should be dried for the minimum time required to remove visible moisture, following the procedure as described by EN ISO 11133.

脚注

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

法的情報

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• ピクトグラム: GHS07
• シグナルワード: Warning
• 危険有害性情報: H317
• 注意書き: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• 危険有害性の分類: Skin Sens. 1
• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3