1.07700

GranuCult^® RAPPAPORT-VASSILIADIS-Soya broth (base)

ISO 6579, for Salmonella spp.

• 別名: Salmonella Enrichment Broth acc. to Rappaport and Vassiliadis, RVS broth (base), Rappaport Vassiliadis Salmonella Enrichment Broth
• UNSPSCコード: 41106213
• NACRES: NA.74

特性

• 認証: ISO 6579
• 品質水準: 200
• フォーム: medium granules (dehydrated (DCM))
• メーカー／製品名: GranuCult^®
• テクニック: microbiological culture: suitable
• pH: 5.0-5.4 (25 °C, 41.8 g/L in H₂O, after autoclaving)
• かさ密度: 700 kg/m³
• アプリケーション: food and beverages; microbiology
• 保管温度: 15-25°C
• 適合性: Salmonella spp.

詳細

詳細

RVS (RAPPAPORT-VASSILIADIS-Soya) broth is a selective medium for the enrichment and isolation of Salmonella. It is a modification of the original Rappaport medium, where soya peptone is used instead of tryptone (peptone from casein) to improve recovery rates of Salmonella. The efficiency of RVS broth for selection of Salmonella is based on (a) the ability of Salmonella sp. to multiply at relatively high osmotic pressures, at relatively low pH values, at a high temperature, and with modest nutritional requirements and (b) the suppression of the toxic effect of malachite green towards Salmonellae in the presence of magnesium chloride.

アプリケーション

RVS (RAPPAPORT-VASSILIADIS-Soya) broth (base) is used for the selective enrichment of Salmonella from food and animal feed, water, and other materials.

アナリシスノート

Appearance (clearness): clear
Appearance (colour): darkblue
pH-value (25 °C): 5.0 - 5.4
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Mixed inoculum of Salmonella, E.coli and P.aeruginosa
Additionaly monoculture of E.coli and E.faecalis
Confirmation on XLD agar resp. Tryptic soy agar
Inoculum on reference medium (Salmonella typhimurium ATCC 14028 (WDCM 00031)): ≤ 100
Inoculum on reference medium (Salmonella enteritidis ATCC 13076 (WDCM 00030)): ≤ 100
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≥ 1000
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)): ≥ 1000
Inoculum on reference medium (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): ≥ 1000
Inoculum on reference medium (Enterococcus faecalis ATCC 29212 (WDCM 00087)): ≥ 1000
Inoculum on reference medium (Enterococcus faecalis ATCC 19433 (WDCM 00009)): ≥ 1000
Growth on XLD agar (Salmonella typhimurium ATCC 14028 (WDCM 00031)): >10 colonies
Growth on XLD agar (Salmonella enteritidis ATCC 13076 (WDCM 00030)): >10 colonies
Colonies (Salmonella typhimurium ATCC 14028 (WDCM 00031)): colourless with black centre
Colonies (Salmonella enteritidis ATCC 13076 (WDCM 00030)): colourless with black centre
Growth on Tryptic soy agar (Escherichia coli ATCC 25922 (WDCM 00013)): <100 colonies
Growth on Tryptic soy agar (Escherichia coli ATCC 8739 (WDCM 00012)): <100 colonies
Growth on Tryptic soy agar (Enterococcus faecalis ATCC 29212 (WDCM 00087)): <10 colonies
Growth on Tryptic soy agar (Enterococcus faecalis ATCC 19433 (WDCM 00009)): <10 colonies
Incubation:
RVS broth: 24 ± 3 hours at 41.5 ± 1 °C aerobic
Confirmation on XLD agar resp. Tryptic Soy Agar: 24 ± 3 hours at 37 ± 1 °C aerobic

脚注

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

法的情報

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 13 - Non Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable