1.00092

FRASER Listeria Ammonium iron (III) Supplement

for Listeria spp., pkg of 10 vials, for the preparation of Half FRASER (Demi FRASER) Broth (Base) or FRASER Broth (Base)

• 別名: FRASER Listeria Ammonium iron(III) Supplement, Listeria Selective Supplement
• 実験式（ヒル表記法）: C₆H₈O₇ · xFe · xH₃N
• CAS番号: 1185-57-5
• MDL番号: MFCD03099410
• UNSPSCコード: 41106212
• EC Index Number: 214-686-6
• NACRES: NA.74

特性

• 認証: ISO 11290; ISO
• 品質水準: 200
• 詳細: GranuCult™ Half FRASER (Demi FRASER) Broth (Base) (1.00025), GranuCult™ FRASER Broth (Base) (1.10398)
• 形状: lyophilized powder
• 有効性: >2000 mg/kg LD₅₀, oral (Rat)
• 組成: Ammonium iron (III) citrate, 250 mg per vial
• 包装: pkg of 10 vials
• テクニック: microbiological culture: suitable
• pH: 6-8 (20 °C, 100 g/L in H₂O)
• 溶解性: 1200 g/L
• アプリケーション: food and beverages; microbiology; pathogen testing
• 保管温度: 15-25°C
• 適合性: Listeria spp.
• InChI: 1S/C6H8O7.Fe.H3N/c7-3(8)1-6(13,5(11)12)2-4(9)10;;/h13H,1-2H2,(H,7,8)(H,9,10)(H,11,12);;1H3/q;+3;/p-3
• InChI Key: FRHBOQMZUOWXQL-UHFFFAOYSA-K

詳細

詳細

For preparation of FRASER broth, two different supplements are needed: FRASER Listeria Selective Supplement and FRASER Listeria Ammonium Iron (III) Supplement. The ammonium iron (III) citrate promotes the growth of the Listeria spp., and together with esculin (a component of the FRASER broth base), permits the β-D-glucosidase detection in Listeria spp.

アプリケーション

FRASER Listeria Ammonium iron (III) Supplement is an additive for the preparation of Half FRASER (Demi FRASER) Broth (Base) or FRASER Broth (Base) for the enrichment of Listeria spp.

関連事項

Replaces: 1000920017

アナリシスノート

Growth promotion test according to ISO 11133
Mixed inoculum of Listeria, E.coli and E.faecalis
Additionaly monoculture of E.coli and E.faecalis
Pre-enrichment in Half Fraser Broth, afterwards enrichment in Fraser Broth
Confirmation on ALOA^®-Medium resp. Tryptic soy agar
Inoculum on reference medium
- Listeria monocytogenes ATCC 19111 (WDCM 00020):
10 - 100
- Listeria monocytogenes ATCC 13932 (WDCM 00021): 10 - 100
- Escherichia coli ATCC 25922 (WDCM 00013): ≥ 1000
- Escherichia coli ATCC 8739 (WDCM 00012): ≥ 1000
- Enterococcus faecalis ATCC 19433 (WDCM 00009): ≥ 1000
- Enterococcus faecalis ATCC 29212 (WDCM 00087): ≥ 1000
Blacking
- Listeria monocytogenes ATCC 19111 (WDCM 00020):
+
- Listeria monocytogenes ATCC 13932 (WDCM 00021): +
- Escherichia coli ATCC 25922 (WDCM 00013): -
- Escherichia coli ATCC 8739 (WDCM 00012): -
- Enterococcus faecalis ATCC 19433 (WDCM 00009): -
- Enterococcus faecalis ATCC 29212 (WDCM 00087): -
Growth on ALOA medium
- Listeria monocytogenes ATCC 19111 (WDCM 00020):
>10 CFU
- Listeria monocytogenes ATCC 13932 (WDCM 00021): >10 CFU
Colonies
- Listeria monocytogenes ATCC 19111 (WDCM 00020):
bluegreen with non transparent zone
- Listeria monocytogenes ATCC 13932 (WDCM 00021): bluegreen with non transparent zone
Growth on Tryptic soy agar
- Escherichia coli ATCC 25922 (WDCM 00013):
no growth
- Escherichia coli ATCC 8739 (WDCM 00012): no growth
- Enterococcus faecalis ATCC 29212 (WDCM 00087): <100 CFU
- Enterococcus faecalis ATCC 19433 (WDCM 00009): <100 CFU
Incubation:
Half Fraser Broth: 24 hours at 30°C, aerobic
Fraser Broth: 48 hours at 37°C, aerobic
Confirmation on ALOA-Medium: 48 hours at 37°C, aerobic
Confirmation on Tryptic soy agar: 24 hours at 35°C, aerobic

法的情報

ALOA is a registered trademark of Biolife Italiana S.r.l.

安全性情報

• 保管分類コード: 13 - Non Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable