07-432
Anti-K+/Cl- Cotransporter (KCC2) Antibody
Upstate®, from rabbit
別名:
Electroneutral potassium-chloride cotransporter 2, Erythroid K-Cl cotransporter 2, Neuronal K-Cl cotransporter, electroneural potassium-chloride cotransporter 2, solute carrier family 12 (potassium-chloride transporter), member 5
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About This Item
UNSPSCコード:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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由来生物
rabbit
品質水準
抗体製品の状態
purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
polyclonal
化学種の反応性
rat, canine, dog
メーカー／製品名
Upstate®
テクニック
immunocytochemistry: suitable
western blot: suitable
アイソタイプ
IgG
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
dry ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
dog ... Slc12A5(485905)
rat ... Slc12A5(171373)
詳細
KCC2 is a member of the cation-chloride cotransporter gene family. It acts as a K-Cl cotransporters. KCCs normally lower intracellular chloride concentrations below the electrochemical equilibrium potential and depending on the chemical concentration gradients of potassium and chloride, KCC2 can operate as a net efflux or influx pathway. It is proposed to act as the main chloride extruder to promote fast hyperpolarizing postsynaptic inhibition in the brain. KCC2 is expressed at high levels in neurons throughout the nervous system and immunofluorescence shows that the protein is localized at inhibitory synapses of the spinal cord. Studies in mice have shown that KCC2 reduces GABA′s inhibitory signaling, resulting in motor defects, epilepsy, and anxiety-like behavior.
特異性
Recognizes rat K+/Cl- Cotransporter (KCC2), Mr ~140 kDa. An unknown band >200 kDa was also detected.
Wide range of species cross-reactivity expected due to sequence homology.
免疫原
N-terminal His-tag fusion protein corresponding to residues 932-1043 of rat K+/Cl- Cotransporter (KCC2).
アプリケーション
Research Category
ニューロサイエンス
ニューロサイエンス
Research Sub Category
イオンチャネル及びトランスポーター
イオンチャネル及びトランスポーター
Immunocytochemistry:
This antibody has been reported by an independent laboratory to detect K+/Cl- Cotransporter (KCC2) in Madin Darby Canine Kidney (MDCK) cells at 1:200 dilution.
This antibody has been reported by an independent laboratory to detect K+/Cl- Cotransporter (KCC2) in Madin Darby Canine Kidney (MDCK) cells at 1:200 dilution.
This Anti-K+/Cl-Cotransporter (KCC2) Antibody is validated for use in IC, WB for the detection of K+/Cl-Cotransporter (KCC2) with more than 15 product citations.
品質
Routinely evaluated by western blot in rat brain membrane preperations.
Western Blot Analysis: 1.0 µg/mL of this lot detected K+/Cl- Cotransporter (KCC2) in rat brain membrane preparations.
Western Blot Analysis: 1.0 µg/mL of this lot detected K+/Cl- Cotransporter (KCC2) in rat brain membrane preparations.
ターゲットの説明
140 kDa
物理的形状
Protein A purified
Format: Purified
Purified rabbit polyclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide and 30% glycerol. Liquid at -20°C.
保管および安定性
Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
アナリシスノート
Control
Rat brain membrane preparation.
Rat brain membrane preparation.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法的情報
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
WGK
WGK 1
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
07-432:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
A Stil et al.
Neuroscience, 164(2), 809-821 (2009-08-25)
The classical GABA/glycine hyperpolarizing inhibition is not observed in the immature spinal cord. GABA(A) and glycine receptors are anions channels and the efficacy of inhibitory transmission in the spinal cord is largely determined by the gradient between intracellular and extracellular
Effects of third trimester-equivalent ethanol exposure on Cl(-) co-transporter expression, network activity, and GABAergic transmission in the CA3 hippocampal region of neonatal rats.
Everett, JC; Licon-Mu?oz, Y; Valenzuela, CF
Alcohol null
J R Williams et al.
The Journal of biological chemistry, 274(18), 12656-12664 (1999-04-23)
The neuron-specific K-Cl cotransporter (KCC2) is hypothesized to function as an active Cl- extrusion pathway important in postsynaptic inhibition mediated by ligand-gated anion channels, like gamma-aminobutyric acid type A (GABAA) and glycine receptors. To understand better the functional role of
MicroRNA-92 modulates K(+) Cl(-) co-transporter KCC2 expression in cerebellar granule neurons.
Barbato, Christian, et al.
Journal of Neurochemistry, 113, 591-600 (2010)
T Q Vu et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 20(4), 1414-1423 (2000-02-09)
The processing of signals by integrative neurons in the retina and CNS relies strongly on inhibitory synaptic inputs, principally from GABAergic and glycinergic neurons that serve primarily to hyperpolarize postsynaptic neurons. Recent evidence indicates that the neuron-specific K-Cl cotransporter 2
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