06-734

Anti-β-Catenin Antibody

Upstate^®, from rabbit

• 別名: catenin (cadherin-associated protein), beta 1 (88kD), catenin (cadherin-associated protein), beta 1 (88kDa), catenin (cadherin-associated protein), beta 1, 88kDa
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 化学種の反応性: human, rat, rabbit, bovine, mouse
• 包装: antibody small pack of 25 μg
• メーカー／製品名: Upstate^®
• テクニック: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• アイソタイプ: IgG
• NCBIアクセッション番号: NP_001091679
• UniProtアクセッション番号: P35222
• 輸送温度: ambient
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... CTNNB1(1499) ; mouse ... Ctnnb1(12387) ; rat ... Ctnnb1(84353)

詳細

詳細

β-catenin plays a central role in cell adhesion, bridging between cadherins and actin cytoskeleton. These activities are regulated through its differential interaction with several molecular partners, including cell-cell adherens junction (AJ) molecules, components of its degradation complex, and transcription factors.
β-Catenin is composed of three domains: a central armadillo repeat domain and two N- and C-terminal tails. β-catenin can be phosphorylated by serine-threonine kinase, such as GSK-3. The phosphorylated β-catenin affects the protein association of β-catenin to E-cadherin and α-cateinin that occurs in the cell. This process can be reversed by de-phosphorylation of β-catenin.

特異性

Recognizes β-Catenin, Mr 92 kDa.

免疫原

KLH-conjugated, synthetic peptide (CGG-SYLDSGIHSGATTTAPSLSGK) corresponding to the consensus GSK3 phosphorylation site of human β -Catenin (amino acids 29-49).

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

Research Sub Category
転写因子

Immunoprecipitation:
5 μg of a previous lot immunoprecipitated β-Catenin from 500 μg of A431 membrane fraction lysate.

Immunocytochemistry:
1-5 μg/mL of a previous lot showed positive immunostaining for β-catenin at cell-cell junctions of A431 cells fixed with 4% paraformaldehyde and permeabilized with 0.25% Triton^™ X-100.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected beta-Catenin in A431 and HUVEC cell lines.

Use Anti-β-Catenin Antibody (Rabbit Polyclonal Antibody) validated in ICC, IP, WB to detect β-Catenin also known as catenin (cadherin-associated protein) beta 1 (88kD).

品質

Routinely evaluated by western blot on A431 total cell lysate.

Western Blot Analysis:
0.1-1 μg/mL of this lot detected β-catenin in an A431 total cell lysate.

ターゲットの説明

92 kDa

物理的形状

Protein A chromatography

Format: Purified

Purified rabbit polyclonal IgG in buffer containing 0.1M Tris Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Prior to removing the cap, centrifuge the vial and gently mix the solution.

アナリシスノート

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.