07-1538

Anti-JMJD4 (NT) Antibody

from rabbit

• 別名: JMJD, Jumonji domain-containing protein 4, jumonji domain containing 4
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 化学種の反応性: human, mouse
• テクニック: ELISA: suitable; immunocytochemistry: suitable; western blot: suitable
• NCBIアクセッション番号: NP_075383.1
• UniProtアクセッション番号: Q9H9V9
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... JMJD4(65094)

詳細

詳細

Covalent modification of histones plays critical role in regulating chromatin structure and transcription. A family of novel JmjC domain-containing histone demethylation (JHDM) enzymes have been identified that remove specific methyl marks from histones. Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate. Contributions of histone demethylase activity to tumor development, decreases in cell proliferation, and hormone-dependent transcriptional activation have been observed.
JMJD4 is a transcription factor that is a putative histone demethylase. It is highly expressed in lymphoid (spleen) and hemopoietic (bone marrow) tissues

特異性

This antibody recognizes the N-terminal region of human JMJD4.

免疫原

Epitope: N-terminus

The immunogen was a KLH-conjugated synthetic peptide corresponding to the N-terminal region of human JMJD4.

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

Research Sub Category
ヒストン修飾タンパク質

エピジェネティクス

ELISA:
This antibody was reported by an outside laboratory to be suitable for ELISA.

Immunocytochemistry:
HeLa and NIH/3T3 cells incubated with anti-JMJD4 (N-terminus) stained positively on nuclei.

Use Anti-JMJD4 (N-terminus) Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC, ELISA to detect JMJD4 (N-terminus) also known as Jumonji domain-containing protein 4.

品質

Western Blot Analysis:
1:250 dilution of this antibody detected JMJD4 on 10 µg of A431 lysate

ターゲットの説明

~53 kDa

物理的形状

Protein G Chromatography

Format: Purified

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

保管および安定性

Stable for 6 months at 2-8°C from date of receipt. For long term storage, store at -20ºC. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

アナリシスノート

Control
A431 lysate

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable