おすすめの製品
由来生物
rabbit
抗体製品の状態
serum
抗体製品タイプ
primary antibodies
クローン
polyclonal
化学種の反応性
human, eukaryotes, Tetrahymena sp.
メーカー／製品名
Upstate®
テクニック
ChIP: suitable
immunocytochemistry: suitable
western blot: suitable
アイソタイプ
IgG
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
acetylation (Penta-acetylation)
遺伝子情報
human ... H4C1(8359)
詳細
Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).
特異性
Expected to cross-react with Tetrahymena and Eukaryote.
Other species not tested.
Recognizes hyperacetylated histone H4, Mr 10 kDa (SDS-PAGE); tri- and tetra-acetylated histone H4 on acid-urea gels. May cross-react with other acetylated proteins.
免疫原
KLH-conjugated, synthetic peptide
(AGGAcKGGAcKGGAcKGGAcKGGAcKGGC) related to amino acids 2 to 19 of Tetrahymena thermophila histone H4.
(AGGAcKGGAcKGGAcKGGAcKGGAcKGGC) related to amino acids 2 to 19 of Tetrahymena thermophila histone H4.
アプリケーション
Research Category
エピジェネティクス及び核内機能分子
エピジェネティクス及び核内機能分子
Research Sub Category
ヒストン
ヒストン
Chromatin Immunoprecipitation:
Reported to immunoprecipitate transcriptionally active chromatin containing acetylated histone H4. (Perry, C.A., 1993)
Immunocytochemistry:
Reported to exclusively stain Tetrahymena macronuclei1 and chromatin in HeLa nuclei. (Perry, C.A., 1993)
Reported to immunoprecipitate transcriptionally active chromatin containing acetylated histone H4. (Perry, C.A., 1993)
Immunocytochemistry:
Reported to exclusively stain Tetrahymena macronuclei1 and chromatin in HeLa nuclei. (Perry, C.A., 1993)
Use Anti-hyperacetylated Histone H4 (Penta) Antibody (Rabbit Polyclonal Antibody) validated in ChIP, ICC, WB to detect hyperacetylated Histone H4 (Penta) also known as H4 histone family member A.
品質
Routinely evaluated by Western Blot on HepG2 lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected HYPERACETYLATED HIS H4 on 10 μg of HepG2 lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected HYPERACETYLATED HIS H4 on 10 μg of HepG2 lysates.
ターゲットの説明
Approx. 10 kDa
物理的形状
Unpurified
Rabbit polyclonal antiserum IgG in buffer containing 30% glycerol.
保管および安定性
Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
アナリシスノート
Control
Tetrahymena cells.
Tetrahymena cells.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法的情報
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Parental nucleosomes segregated to newly replicated chromatin are underacetylated relative to those assembled de novo.
Perry, C A, et al.
Biochemistry, 32, 13615-13623 (1993)
A network connecting Runx2, SATB2, and the miR-23a~27a~24-2 cluster regulates the osteoblast differentiation program.
Hassan, MQ; Gordon, JA; Beloti, MM; Croce, CM; van Wijnen, AJ; Stein, JL; Stein, GS; Lian, JB
Proceedings of the National Academy of Sciences of the USA null
The PB2 subunit of the influenza virus RNA polymerase affects virulence by interacting with MAVS and inhibiting IFN-{beta} expression.
Graef KM, Vreede FT, Lau YF, McCall AW, Carr SM, Subbarao K, Fodor E
Journal of virology null
Evidence for monomeric actin function in INO80 chromatin remodeling.
Kapoor, P; Chen, M; Winkler, DD; Luger, K; Shen, X
Nature Structural and Molecular Biology null
Cindy Law et al.
The Journal of biological chemistry, 290(21), 13234-13249 (2015-04-04)
H2A.Z is a histone H2A variant that is essential for viability in Tetrahymena and Drosophila and also during embryonic development of mice. Although implicated in diverse cellular processes, including transcriptional regulation, chromosome segregation, and heterochromatin formation, its essential function in
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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