05-669
Anti-phospho-Akt1/PKBα (Ser473) Antibody, clone 11E6
clone 11E6, Upstate®, from mouse
別名:
Protein kinase B, RAC-alpha serine/threonine-protein kinase, murine thymoma viral (v-akt) oncogene homolog-1, rac protein kinase alpha, v-akt murine thymoma viral oncogene homolog 1
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About This Item
UNSPSCコード:
12352203
eCl@ss:
32160702
NACRES:
NA.41
由来生物
mouse
品質水準
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
クローン
11E6, monoclonal
化学種の反応性
rat, canine, mouse, human
メーカー／製品名
Upstate®
テクニック
ELISA: suitable
western blot: suitable
アイソタイプ
IgG1κ
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
dry ice
ターゲットの翻訳後修飾
phosphorylation (pSer473)
遺伝子情報
dog ... Akt1(490878)
human ... AKT1(207)
詳細
The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKB-β or RacPK-β) and Akt3 (also designated PKB-γ or thyoma viral proto-oncogene 3), which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. All members of the Akt family have a Pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-β tyrosine residues 740 and 751, which bind the 85 kDa subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1(IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Phosphorylation of both residues is important to generate a high level of Akt1 activity, and the phosphorylation of Thr 308 is not dependent on phosphorylation of Ser 473 in vivo. Thus, Akt proteins become phosphorylated and activated in insulin/IGF-1- stimulated cells by an upstream kinase(s). The activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin. Taken together, this data strongly suggests that the protein signals downstream of the PI kinases.
特異性
Recognizes phosphorylated Akt1/PKBα at Ser473, Mr 60 kDa. This lot of antibody does not cross-react with Akt1/PKBα dephosphorylated at Ser473.
アプリケーション
ELISA:
A previous lot was recommended at 0.05 mg/mL by an independent laboratory for ELISA.
A previous lot was recommended at 0.05 mg/mL by an independent laboratory for ELISA.
This Anti-Akt1 Antibody, clone 11E6 is validated for use in ELISA, WB for the detection of phospho-Akt1/PKBα (Ser473).
品質
Evaluated by western blot on lysates from mouse NIH-3T3 fibroblasts treated with PDGF.
Western Blot Analysis: 0.5-2 µg/mL of this antibody detected phosphorylated Akt1/PKBa in lysates from mouse NIH-3T3 fibroblasts treated with 100 ng/mL PDGF for 20 minutes.
Western Blot Analysis: 0.5-2 µg/mL of this antibody detected phosphorylated Akt1/PKBa in lysates from mouse NIH-3T3 fibroblasts treated with 100 ng/mL PDGF for 20 minutes.
ターゲットの説明
60 kDa
物理的形状
Format: Purified
Purified mouse monoclonal IgG1κ lyophilized from 1 mL 2X PBS, PEG, sucrose, and 0.09% sodium azide.
Reconstitute with 1 mL H2O (15 min. at RT). Concentration after reconstitution: 100 µg/mL
Reconstitute with 1 mL H2O (15 min. at RT). Concentration after reconstitution: 100 µg/mL
保管および安定性
Stable for 1 year at -20°C from date of receipt.
Reconstitute prior to use. Reconstitute with 1 mL H2O for 15 min at room temperature. Reconstituted aliquots should be stored frozen at -80°C. Thawed aliquots may be stored at 2-8°C for up to 3 months. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Reconstitute prior to use. Reconstitute with 1 mL H2O for 15 min at room temperature. Reconstituted aliquots should be stored frozen at -80°C. Thawed aliquots may be stored at 2-8°C for up to 3 months. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
アナリシスノート
Control
PDGF treated NIH-3T3 cell lysates.
PDGF treated NIH-3T3 cell lysates.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法的情報
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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シグナルワード
Danger
危険有害性情報
危険有害性の分類
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3
保管分類コード
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 1
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
毒物及び劇物取締法
毒物
労働安全衛生法名称等を表示すべき危険物及び有害物
名称等を表示すべき危険物及び有害物
労働安全衛生法名称等を通知すべき危険物及び有害物
名称等を通知すべき危険物及び有害物
Jan Code
05-669:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Neuroprotective effects of activated protein C through induction of insulin-like growth factor-1 (IGF-1), IGF-1 receptor, and its downstream signal phosphorylated serine-threonine kinase after spinal cord ischemia in rabbits.
Yamauchi, T; Sakurai, M; Abe, K; Takano, H; Sawa, Y
Stroke null
Akt1 deficiency modulates reward learning and reward prediction error in mice.
Y-C Chen,Y-W Chen,Y-F Hsu,W-T Chang,C K Hsiao,M-Y Min,W-S Lai
Genes, Brain, and Behavior null
P21 (waf1/cip1) is required for non-small cell lung cancer sensitive to Gefitinib treatment.
Zhao YF, Wang CR, Wu YM, Ma SL, Ji Y, Lu YJ
Biomedicine and Pharmacotherapy null
Yoshikazu Matsuoka et al.
Stem cells (Dayton, Ohio), 29(11), 1783-1791 (2011-09-08)
Although c-kit is expressed highly on murine hematopoietic stem cells (HSCs) and essential for bone marrow (BM) hematopoiesis, the significance of the high level of expression of c-kit on HSCs was not well determined. We show here that CD150(+) CD48(-)
Activated protein C has a protective effect against myocardial I/R injury by improvement of endothelial function and activation of AKT1.
Maehata, Y; Miyagawa, S; Sawa, Y
Testing null
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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