05-389-AF488

Anti-Rac1 Antibody, clone 23A8, Alexa Fluor^™ 488 Conjugate

clone 23A8, from mouse, ALEXA FLUOR^™ 488

• 別名: Ras-related C3 botulinum toxin substrate 1, Cell migration-inducing gene 5 protein, Ras-like protein TC25, p21-Rac1, Rac1
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 結合体: ALEXA FLUOR^™ 488
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: 23A8, monoclonal
• 化学種の反応性: rat, mouse, human
• テクニック: immunocytochemistry: suitable
• アイソタイプ: IgG2b
• NCBIアクセッション番号: NM_006908
• UniProtアクセッション番号: P63000
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... RAC1(5879)

詳細

詳細

Ras-related C3 botulinum toxin substrate 1 (UniProt P63000; also known as Cell migration-inducing gene 5 protein, p21-Rac1, Rac-1, Ras-like protein TC25, TC-25) is encoded by the RAC1 (also known as TC25) gene (ORF MIG5; Gene ID 5879) in human. Rac1, RhoA, and Cdc42 (UniProt P63000, P61586, and P60953, respectively) are among the most studied Rho family of small GTPases that are involved in a wide variety of cellular functions, including cell polarity, vesicular trafficking, and cell cycle by regulating intracellular actin dynamics. In mammals, the Rho family consists of 22 members, which can be further divided into the Rac subfamily (Rac1, Rac2, Rac3, and RhoG), Cdc42 subfamily (Cdc42, TC10, TCL, Chip, and Wrch-1), RhoA subfamily (RhoA, RhoB, and RhoC) and other Rho-GTPases (RhoE/Rnd3, RhoH/TTF, Rif, RhoBTB1, RhoBTB2, Miro-1, Miro-2, RhoD, Rnd1, and Rnd2). Akt is reported to negatively regulate mixed-lineage kinase 3 (MLK3) by phosphorylating Rac1 at Ser71.

特異性

Recognizes Rac1; Strong Rac1 preference with slight Rac2 cross-reactivity.

免疫原

Recombinant protein containing the full length human Rac.

アプリケーション

Research Category
細胞シグナル伝達

Research Sub Category
発生シグナル伝達

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Rac1 in NIH/3T3 cells.
The unconjugated antibody (Cat. No. 05-389) is shown to be suitable also for Western blotting, immunohistochemistry, and immunoprecipitation applications.

This Anti-Rac1 Antibody, clone 23A8, Alexa Fluor^™ 488 Conjugate is validated for use in Immunocytochemistry for the detection of Rac1.

品質

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Rac1 in A431 cells.

ターゲットの説明

~21 kDa observed

物理的形状

Protein G Purified

Purified mouse antibody conjugate in PBS with 15 mg/ml BSA and 0.1% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

法的情報

ALEXA FLUOR is a trademark of Life Technologies

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable