05-235

Anti-EF1α Antibody, clone CBP-KK1

clone CBP-KK1, Upstate^®, from mouse

• 別名: CTCL tumor antigen, EF1a-like protein, Elongation factor 1 A-1, Elongation factor Tu, Leukocyte receptor cluster member 7, cervical cancer suppressor 3, elongation factor 1 alpha subunit, elongation factor 1-alpha, eukaryotic translation elongation facto
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: CBP-KK1, monoclonal
• 化学種の反応性: bacteria, Saccharomyces cerevisiae
• 化学種の反応性（ホモロジーによる予測）: mammals
• メーカー／製品名: Upstate^®
• テクニック: immunoprecipitation (IP): suitable; western blot: suitable
• アイソタイプ: IgG1κ
• NCBIアクセッション番号: NM_006620
• UniProtアクセッション番号: P68104
• 輸送温度: dry ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... EEF1A1(1915)

詳細

詳細

Human eukaryotic translation elongation factor 1 alpha has two isoforms, alpha 1 and alpha 2. The alpha 1 isoform gene encodes a subunit of the elongation factor-1 complex which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. The alpha 1 isoform is expressed in brain, placenta, lung, liver, kidney, and pancreas. The other isoform (alpha 2) is expressed in brain, heart and skeletal muscle.
The alpha 1 isoform is identified as an autoantigen in 66% of patients with Felty syndrome. The gene for the alpha 1 isoform has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. The gene for the alpha 2 may be critical in the development of ovarian cancer.

特異性

This antibody recognizes EF-1α (Elongation factor-1 alpha), Mr 53 kDa. Occasionally, a 38 kDa protein is also detected in A431 cell lysate.

免疫原

Crude calmodulin-binding proteins from Trypanosoma brucei isolated using calmodulin-affinity chromatography. Clone CBP-KK1.

アプリケーション

Research Category
タンパク質輸送

Research Sub Category
RNA代謝及び結合タンパク質

RNA結合タンパク質（RBP）

Detect EF1α using this Anti-EF1α Antibody, clone CBP-KK1 validated for use in IP & WB.

Immunoprecipitation:
4 µg of a previous lot immunoprecipitated EF1α from an A431 RIPA cell lysate.

品質

Routinely evaluated on RIPA lysates from non-stimulated A431 cells, human Jurkat or mouse 3T3/A31.

Western Blot Analysis:
0.5-2 µg/mL of this lot detected EF1α in RIPA lysates from non-stimulated A431 cells. Previous lots detected EF1α in human Jurkat and mouse 3T3/A31 RIPA cell lysates.

ターゲットの説明

53 kDa

物理的形状

Protein G Chromatography

Format: Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

アナリシスノート

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable