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安全性情報

03-179

Sigma-Aldrich

RIPAb+ SUZ12 - RIP Validated Antibody and Primer Set

from mouse

別名:

ChET 9 protein, Chromatin precipitated E2F target 9 protein, Joined to JAZF1 protein, Suppressor of zeste 12 protein homolog, joined to JAZF1, suppressor of zeste 12 homolog (Drosophila)

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About This Item

UNSPSCコード:
12352203
eCl@ss:
32160702
NACRES:
NA.52

由来生物

mouse

品質水準

クローン

monoclonal

化学種の反応性

human

メーカー/製品名

RIPAb+
Upstate®

テクニック

RIP: suitable
immunoprecipitation (IP): suitable
western blot: suitable

アイソタイプ

IgG1κ

NCBIアクセッション番号

UniProtアクセッション番号

輸送温度

dry ice

遺伝子情報

human ... SUZ12(23512)

詳細

SUZ12 is a polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. They probably act via the methylation of histones, rendering chromatin heritably changed in its expressibility. Component of the PRC2 complex, which methylates ′Lys-9′ and ′Lys-27′ residues of histone H3.

特異性

SUZ12

免疫原

Full length, recombinant human SUZ12.

アプリケーション

Research Category
エピジェネティクス及び核内機能分子
Research Sub Category
エピジェネティクス
Immunoprecipitation from RIP lysate:
Representative lot data.
RIP lysate from HeLa cells (~2 X 10E7 cell equivalents per IP) was subjected to immunoprecipitation using either 5 µg of a normal mouse IgG (Cat. #CS200621), or 5 µg of Anti-SUZ12 antibody (Cat. # CS203201).
20 percent of the precipitated proteins (lane 1: mouse IgG, lane 2: SUZ12) were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-SUZ12 antibody (Cat. # CS203201, 1:1000). Proteins were visualized using One-Step IP-Western kit (GenScript Cat. # L00232)
Arrow indicates SUZ12 (Figure 2).

Western Blot Analysis:
Representative lot data.
Lysates from HeLa cells were resolved by electrophoresis, transferred to PVDF and probed with anti-SUZ12 (1:1,000 dilution).
Proteins were visualized using goat anti-mouse secondary antibody conjugated to HRP and chemiluminescence detection.
Arrow SUZ12 (~95 kDa) (Figure 3).
This RIPAb+ SUZ12 -RIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.

包装

10 assays per set. Recommended use: ~5 μg of antibody per RIP (dependent upon biological context).

品質

RNA Binding Protein Immunoprecipitation:
RIP Lysate prepared from HeLa cells (2 X 10E7 cell equivalents per IP) were subjected to immunoprecipitation using either 5 µg of a normal mouse IgG , or 5 µg of Anti-SUZ12 antibody and the Magna RIP® RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700).
Successful immunoprecipitation of SUZ12-associated RNA was verified by qPCR using RIP Primers lincSFPQ, (Figure 1)
Please refer to the Magna RIP (Cat. # 17-700) or EZ-Magna RIP (Cat. # 17-701) protocol for experimental details.

ターゲットの説明

95 kDa

物理的形状

Protein A purified
Anti-SUZ12 (Mouse Monoclonal) -
One vial containing 50 μg of protein A purified antibody in PBS containing 0.1% azide, before the addition of 30% glycerol. Store at -20°C.

Normal Mouse IgG -
One vial containing 125 µg of purified mouse IgG in 125 µL of storage buffer containing 0.1% sodium azide. Store at -20°C.

RIP Primers, lincSFPQ -
One vial containing 75 μL of 5 μM of each primer specific for the human lincRNA SFPQ. Store at -20°C.
FOR: TCG TAC TGT TAG GCC CTT GG
REV: AAC CTT GCA TGA AGA GCA CC
Format: Purified

保管および安定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

アナリシスノート

Control
Includes negative control normal mouse IgG antibody and control primers specific for the cDNA of human lincRNA SFPQ.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

MAGNA RIP is a registered trademark of Merck KGaA, Darmstadt, Germany
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

保管分類コード

10 - Combustible liquids


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

03-179:


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Felipe C Beckedorff et al.
PLoS genetics, 9(8), e1003705-e1003705 (2013-08-31)
The down-regulation of the tumor-suppressor gene RASSF1A has been shown to increase cell proliferation in several tumors. RASSF1A expression is regulated through epigenetic events involving the polycomb repressive complex 2 (PRC2); however, the molecular mechanisms modulating the recruitment of this
Fuzhen Qi et al.
Journal of hematology & oncology, 10(1), 48-48 (2017-02-18)
Long noncoding RNAs (lncRNAs) have emerged as important regulators of tumorigenesis and cancer progression. Recently, the lncRNA AGAP2-AS1 was identified as an oncogenic lncRNA in human non-small cell lung cancer (NSCLC) and its elevated expression was linked to NSCLC development

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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