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N3755

Sigma-Aldrich

Nuclease micrococcal from Staphylococcus aureus

100-300 units/mg protein

Sinonimo/i:

Micrococcal nuclease, Endonuclease micrococcal, MNase, Micrococcal endonuclease

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About This Item

Numero CAS:
9013-53-0
Classificazione EC (Enzyme Commission):
3.1.31.1 (BRENDA, IUBMB)
Numero CE:
232-748-0
Numero MDL:
MFCD00131731
Codice UNSPSC:
12352204
NACRES:
NA.54

Origine biologica

Staphylococcus aureus

Livello qualitativo

300

Forma fisica

lyophilized powder

Attività specifica

100-300 units/mg protein

Composizione

Protein, ≥40% E1%/280 (Balance primarily sodium citrate)

tecniche

DNA purification: suitable

Compatibilità

suitable for molecular biology

applicazioni

diagnostic assay manufacturing

Temperatura di conservazione

−20°C

Informazioni sul gene

Staphylococcus aureus subsp. aureus Mu50 ... nuc(1120790)

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Categorie correlate

Applicazioni

Nuclease from Staphylococcus aureus has been used in a study to assess coagulase and heat-resistant strains found in animals. It has also been used in a study to investigate the expression characteristic of two genes in S. aureus that encode two thermostable nucleases.

Azioni biochim/fisiol

Cleavage of DNA by Nuclease micrococcal is greater at the 5′ sides of A or T nucleotides than at G or C.
Hydrolyzes 5′-phosphodiester bonds of DNA.

Definizione di unità

One unit will produce 1.0 μmole of acid soluble polynucleotides from native DNA per min at pH 8.8 at 37 °C, based on EM/260 = 10,000 for the mixed nucleotides.

Altre note

Note: One μmolar unit = approx. 85 A260 units, where an A260 unit is equivalent to a ΔA260 of 1.0 in 30 min at pH 8.8 at 37 °C in a 3.55 mL reaction volume. Light path = 1 cm.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

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Journal of Applied Microbiology, 39, 197-207 (1975)
Yu Hu et al.
Foodborne pathogens and disease, 9(3), 265-271 (2012-03-03)
Thermonuclease is known as a specific virulence factor in Staphylococcus aureus. It is widely used as a genetic marker for detection of S. aureus in various types of food. Previous studies have revealed the existence of two functional thermostable nucleases
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The interplay between chromatin structure and DNA topology is a fundamental, yet elusive, regulator of genome activities. A paradigmatic case is the "linking number paradox" of nucleosomal DNA, which refers to the incongruence between the near two left-handed superhelical turns
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