10107921001
Roche
Nuclease S7
Micrococcal nuclease, from Staphylococcus aureus
Sinonimo/i:
s7 nuclease
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About This Item
Codice UNSPSC:
12352204
Prodotti consigliati
Origine biologica
Staphylococcus aureus
Livello qualitativo
Forma fisica
lyophilized
Attività specifica
~15,000 U/mg
Confezionamento
pkg of 15,000 U
Produttore/marchio commerciale
Roche
pH ottimale
7.5
Condizioni di spedizione
wet ice
Applicazioni
- Removal of endogenous RNA from in vitro translation systems
- RNA sequencing experiments
Nuclease S7 has been used in ribonuclease selection process for ribosome profiling.
Azioni biochim/fisiol
Nuclease S7 is an endonuclease that mainly cleaves single-stranded substrates. It also cleaves double-stranded DNA or RNA. It cleaves the 5′-phosphodiester bonds of RNA and DNA to yield 3′-mononucleotides and oligonucleotides. Activity of the enzyme is dependent on Ca2+ and can be completely inactivated by the addition of EDTA. Nuclease S7 cleavage is highly specific to AT rich region than GC on the DNA.
Definizione di unità
One unit releases a sufficient amount of acid-soluble oligonucleotides to produce an increase of 1.0 at A260.
Stoccaggio e stabilità
Store unopened reagent at 2-8 °C; when diluted to 1 mg/mL, store at -15 to -25 °C.
Altre note
For life science research only. Not for use in diagnostic procedures.
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
Organi bersaglio
Respiratory system
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
does not flash
Punto d’infiammabilità (°C)
does not flash
Certificati d'analisi (COA)
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