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N1531

Sigma-Aldrich

Denaturation Solution

for Northern and Southern blotting

Sinonimo/i:

Denaturation Solution For Blotting Technique, Denaturation Solution for Neutral Southern Transfer

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About This Item

Codice UNSPSC:
12161700
NACRES:
NA.25

Grado

for molecular biology

Livello qualitativo

200

Forma fisica

solution

Temperatura di conservazione

room temp

Descrizione generale

Sigma′s denaturing solution is a strong base that denatures double-stranded DNA so that it can be efficiently transferred to nylon membrane and subsequently hybridized to labeled probe. When used with a depurination solution, treatment results in the hydrolysis of the phosphodiester backbone at the sites of depurination, allowing for more efficient transfer of large DNA in neutral Southern transfers.

Applicazioni

Denaturation Solution is suitable for denaturing dsDNA before the transfer to membranes during Northern and Southern blotting procedures.

Componenti

The denaturing solutions is composed of 0.5N NaOH, and 1.5M NaCl.

Altre note

0.5 N NaOH, 1.5 M NaCl

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Pittogrammi

Corrosion
GHS05

Avvertenze

Danger

Indicazioni di pericolo

H290,H314

Classi di pericolo

Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B

Codice della classe di stoccaggio

8B - Non-combustible corrosive hazardous materials

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

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Protocolli

Northern and Southern Blot Protocols & Introduction

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

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