D9905
Denhardt′s Solution
for Northern and Southern blotting, lyophilized powder
Sinonimo/i:
Denhardt′s Solution For Blotting Technique
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About This Item
Codice UNSPSC:
12161700
NACRES:
NA.25
Prodotti consigliati
Grado
for molecular biology
Livello qualitativo
Forma fisica
lyophilized powder
tecniche
Southern blotting: suitable
Attività estranea
DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected
Temperatura di conservazione
2-8°C
Descrizione generale
Denhardt′s Solution is a blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. This powder can be used to make a 50x concentrate that can be diluted to any concentration for use in a variety of pre-hybridization and hybridization buffers.
Applicazioni
Denhardt′s Solution has been used:
- as a component in hybridization buffer for the hybridization of brain sections
- in in-situ hybridization peroxidase-(antiperoxidase method-whole mounts) (ISHPAP-WM) process
- in the preparation of hybridization mixture
Suitable as a blocking Buffer for Southern and Northern blot membranes.
Azioni biochim/fisiol
Denhardt′s solution and gelatin-chrome alum can be used to treat chromosomes for G-banding with trypsin after in situ hybridization.
Componenti
Each vial of Denhardt′s powder contains 50mg Bovine Serum Albumin (BSA), 50mg Ficoll, and 50mg Polyvinylpyrrolidone (PVP).
Nota sulla preparazione
To make a 50x concentrate, dissolve entire contents of vial in 5 mL molecular biology grade water. Store at -20°C for up to two years.
Prodotti correlati
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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