11207733910
Roche
Anti-Digoxigenin-POD, Fab fragments
from sheep
Sinonimo/i:
anti-digoxigenin, digoxigenin
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About This Item
Codice UNSPSC:
12352203
Prodotti consigliati
Origine biologica
sheep
Livello qualitativo
Coniugato
peroxidase conjugate
Forma dell’anticorpo
F(ab′)2 fragment of affinity isolated antibody
Tipo di anticorpo
primary antibodies
Clone
polyclonal
Forma fisica
lyophilized (clear, brown solution after reconstitution)
Confezionamento
pkg of 150 U
Produttore/marchio commerciale
Roche
Temperatura di conservazione
2-8°C
Descrizione generale
Digoxigenin is a hapten, useful in labeling nucleic acids and in detection systems. This product contains Fab fragments from an anti-digoxigenin antibody, conjugated with horse-radish peroxidase (POD). Anti-Digoxigenin-POD, Fab fragments is useful for the detection of digoxigenin-labeled compounds.
Specificità
The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.
Applicazioni
Use Anti-Digoxigenin-POD, Fab fragments for the detection of digoxigenin-labeled compounds using:
- Dot blot
- ELISA
- Immunohistocytochemistry
- In situ hybridization
- Western blot
Nota sulla preparazione
After immunization with digoxigenin, sheep IgG was purified by ion-exchange chromatography, and the specific IgG was isolated by immunosorption. The Fab fragments obtained by papain digestion were purified by gel filtration, conjugated to the specific label, and stabilized in buffer.
Working concentration: Working concentration of conjugate will depend on the application and substrate. The following concentrations should be taken as a guideline: Dot blot: 150 mU/ml
Working solution: 100 mM Tris-HCl, 150 mM NaCl, pH 7.5.
1% Blocking reagent (w/v), 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding.
Working concentration: Working concentration of conjugate will depend on the application and substrate. The following concentrations should be taken as a guideline: Dot blot: 150 mU/ml
- ELISA: 50 to 150 mU/ml
- Immunohistocytochemistry: 250 to 500 mU/ml
- In situ hybridization: 1.5 to 7.5 U/ml
- Southern blot: 150 mU/ml
- Western blot: 250 to 500 mU/ml
Working solution: 100 mM Tris-HCl, 150 mM NaCl, pH 7.5.
1% Blocking reagent (w/v), 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding.
Ricostituzione
Add 1 ml double-distilled water to a final concentration of 150 U/ml.
Altre note
For life science research only. Not for use in diagnostic procedures.
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Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Skin Sens. 1
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
does not flash
Punto d’infiammabilità (°C)
does not flash
Certificati d'analisi (COA)
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Articoli
Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.
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