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Key Documents

D8156

Sigma-Aldrich

Monoclonal Anti-Digoxin antibody produced in mouse

clone DI-22, ascites fluid

Sinonimo/i:

Monoclonal Anti-Digoxin

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About This Item

Codice UNSPSC:
12352203
NACRES:
NA.43

Origine biologica

mouse

Coniugato

unconjugated

Forma dell’anticorpo

ascites fluid

Tipo di anticorpo

primary antibodies

Clone

DI-22, monoclonal

contiene

15 mM sodium azide

tecniche

dot blot: suitable
flow cytometry: suitable
indirect ELISA: 1:10,000 using digoxin-BSA
indirect ELISA: 1:2,500 using digoxigenin-transferrin

Isotipo

IgG1

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

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Descrizione generale

Monoclonal Anti-Digoxin (mouse IgG1 isotype) is derived from the hybridoma DI-22 produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with digoxin- keyhole limpet hemocyanin (KLH). Digoxin is obatained from the leaves of the Digitalis lanata, a foxglove plant. The digoxin molecule is made up of a sugar and a cardenolide. It has a molecular weight is 780.95 Da. It is a odorless white crystal, which is soluble in alcohol and freely soluble in pyridine. It is expressed in the duodenum, kidneys, liver and the blood-brain barrier.

Specificità

Antibody is specific for digoxin and shows a high affinity for digoxigenin.
Monoclonal Anti-Digoxin antibody is specific for digoxin and digoxin-labeled compounds, and shows strong cross-reactivity with digoxigenin.

Immunogeno

Digoxin-KLH.

Applicazioni

Monoclonal Anti-Digoxin antibody produced in mouse has been used in:
  • enzyme-linked immunosorbent assay (ELISA)
  • dot blot
  • flow cytometry
  • fluorescence in situ hybridization (FISH)
  • DNA hybridization
  • in-situ hybridization (ISH)
  • immunodetection of the probe and chromosome X

Azioni biochim/fisiol

Digoxin is a phytoestrogen that binds estrogen receptors and produces inotropic effects in cardiac muscle. Studies show that digoxin decreases the risk of breast and uterus cancers that are estrogen sensitive.
Digoxin is a substrate for P-glycoprotein (P-gp), a membrane efflux transporter. This antibody may be used to detect digoxin-labeled compounds such as oligonucleotides, antibodies or peptides.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

nwg

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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Hesed M Padilla-Nash et al.
Nature protocols, 1(6), 3129-3142 (2007-04-05)
Classical banding methods provide basic information about the identities and structures of chromosomes on the basis of their unique banding patterns. Spectral karyotyping (SKY), and the related multiplex fluorescence in situ hybridization (M-FISH), are chromosome-specific multicolor FISH techniques that augment
GSTT1-dependent induction of centromere-negative and-positive micronuclei by l, 2: 3, 4-diepoxybutane in cultured human lymphocytes
Vlachodimitropoulos D, et al.
Mutagenesis, 12(5), 397-403 (1997)
Waqar Ahmed et al.
Scientific reports, 8(1), 15438-15438 (2018-10-20)
Epstein-Barr virus-encoded RNAs (EBER1 and EBER2) are two highly abundant, non-protein coding RNAs consistently expressed in all EBV infected cells, but their function remains poorly understood. Conventional in situ hybridization studies have indicated that these RNAs are present exclusively in
Ying-Liang Duan et al.
Journal of virology, 88(7), 3861-3873 (2014-01-24)
After infection, human cytomegalovirus (HCMV) persists for life. Primary infections and reactivation of latent virus can both result in congenital infection, a leading cause of central nervous system birth defects. We previously reported long-term HCMV infection in the T98G glioblastoma
Digoxin
Small Animal Clinical Pharmacology, 805-806 (2009)

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