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B6429

Sigma-Aldrich

Casein Blocking Buffer 10x

for Northern and Southern blotting, solution

Sinonimo/i:

10x Blocking Buffer

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About This Item

Codice UNSPSC:
12161700
NACRES:
NA.54

Grado

for molecular biology

Livello qualitativo

200

Forma fisica

solution

tecniche

Southern blotting: suitable

Temperatura di conservazione

room temp

Categorie correlate

Descrizione generale

Casein is a phosphoprotein found in milk. It is classified into four subtypes, including αS1-, αS2-, β-, and κ-caseins.
Sigma’s 10x Blocking Buffer is a casein-based blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes.

Applicazioni

Casein Blocking Buffer 10x has been used in:

  • western blotting
  • enzyme linked immunosorbent assay (ELISA)
  • immunohistochemistry

Caratteristiche e vantaggi

  • Better blocking performance than dried milk or BSA
  • Easy-to-use concentrate
  • Compatible with neutral and positively-charged membranes
  • Compatible with chemiluminescent and colorimetric detection assays

Nota sulla preparazione

Dilute 10x Blocking Buffer with molecular biology grade water before use.

Pittogrammi

Exclamation markEnvironment
GHS07,GHS09

Avvertenze

Warning

Indicazioni di pericolo

H315,H317,H319,H411

Classi di pericolo

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Protocolli

10x Blocking Buffer Protocol

Membrane-based blotting applications that employ enzyme conjugates to generate colorimetric or chemiluminescent signal require the use of an added blocking step to decrease the signal generated by non-specific binding.

Northern and Southern Blot Protocols & Introduction

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

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