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B6185

Sigma-Aldrich

Bionic™ Buffer, 10X Concentrate

for Northern and Southern blotting, solution

Sinonimo/i:

Sodium Borate Electrophoresis Buffer

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About This Item

Codice UNSPSC:
41105319
NACRES:
NA.25

Grado

for molecular biology

Livello qualitativo

200

Descrizione

Mode of use Voltage of 150 V is recommended for small electrophoresis chambers while a mid to large apparatus may be run at 200-250 V. User should test their chamber to determine optimum voltage.

Forma fisica

solution

Concentrazione

10 ×

Impurezze

DNase, RNase and nickase, none detected

pH

8.0-8.5 (20-25 °C)

Temperatura di conservazione

room temp

Descrizione generale

Bionic Buffer is a unique alternative to traditional TBE (TRIS borate-EDTA) and TAE (TRIS acetate-EDTA) electrophoresis buffers. Bionic Buffer allows for higher voltage runs, extremely rapid running times, and high-resolution band separation.

Applicazioni

Suitable for:
  • Use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures
  • Use with TBA and TAE gels
  • Preparation of agarose gels

Caratteristiche e vantaggi

  • Faster run times
  • Sharper bands
  • Easy to dilute and use
  • Flexible use with a variety of gel types

Confezionamento

The 4 L and 10 L sizes are supplied in dispensers with a spigot.

Avvertenza

Test electrophoresis chambers to determine highest voltage. Voltages beyond the recommended range for your apparatus may result in blown fuses or damage to your apparatus.

Note legali

Bionic is a trademark of Sigma-Aldrich Co. LLC

Pittogrammi

Health hazardExclamation mark
GHS08,GHS07

Avvertenze

Danger

Indicazioni di pericolo

H319,H360FD

Classi di pericolo

Eye Irrit. 2 - Repr. 1B

Codice della classe di stoccaggio

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Protocolli

Northern and Southern Blot Protocols & Introduction

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.