Passa al contenuto
Merck
Tutte le immagini(1)

Key Documents

M9943

Sigma-Aldrich

10X MTP Taq Buffer

10X buffer, free of DNA contaminants; use with MTP Taq DNA Polymerase

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali


About This Item

Codice UNSPSC:
41106306
NACRES:
NA.52

Livello qualitativo

Forma fisica

liquid

Colore

colorless

Condizioni di spedizione

wet ice

Temperatura di conservazione

−20°C

Descrizione generale

DNA contaminants can be introduced into PCR through a number of reagents. To minimize the risk of contaminant DNA during PCR, we offer 10x MTP Taq buffer to be used with MTP Taq DNA Polymerase (Product No. D7442). Each lot of MTP Taq buffer undergoes strict quality control testing to ensure the absence of contaminating DNA. To prevent false positive PCR results, only DNA-free reagents should be used in PCR reactions with MTP Taq DNA polymerase.

Applicazioni

10X MTPTaq Buffer has been used:

  • for the amplification of bacterial 16s rRNA genes from purified DNA
  • to amplify archaeal 16s rRNA
  • along with MTP Taq DNA polymerase (D7442) to amplify specific bacterial 16s rRNA genes from purified DNA

Note legali

MTP is a trademark of Sigma-Aldrich Co. LLC

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

I clienti hanno visto anche

Florie Maillard et al.
Cells, 8(1) (2019-01-13)
Crohn's disease is characterized by abnormal ileal colonization by adherent-invasive E. coli (AIEC) and expansion of mesenteric adipose tissue. This study assessed the preventive effect of spontaneous physical activity (PA) on the gut-adipose tissue in a mouse model that mimics
Risk assessment models and contamination management: implications for broad-range ribosomal DNA PCR as a diagnostic tool in medical bacteriology.
B Cherie Millar et al.
Journal of clinical microbiology, 40(5), 1575-1580 (2002-05-01)
Florie Maillard et al.
Cells, 8(1) (2019-01-13)
Crohn's disease is characterized by abnormal ileal colonization by adherent-invasive E. coli (AIEC) and expansion of mesenteric adipose tissue. This study assessed the preventive effect of spontaneous physical activity (PA) on the gut-adipose tissue in a mouse model that mimics
C E Corless et al.
Journal of clinical microbiology, 38(5), 1747-1752 (2000-05-02)
A set of universal oligonucleotide primers specific for the conserved regions of the eubacterial 16S rRNA gene was designed for use with the real-time PCR Applied Biosystems 7700 (TaqMan) system. During the development of this PCR, problems were noted with

Protocolli

Method for bacterial genome analysis and detection of pathogens. Minimize false positive PCRs through lab design and reagents tested for use in bacterial PCR applications.

Method for PCR amplification of Bacterial DNA with low contamination using MTP Taq DNA Polymerase (D7442)

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.