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D4309

Sigma-Aldrich

REDTaq® DNA Polymerase

Taq for routine PCR with inert dye, 10X buffer included

Sinonimo/i:

DNA polymerase with loading dye, Taq DNA polymerase

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About This Item

Numero MDL:
MFCD01635818
Codice UNSPSC:
12352204
NACRES:
NA.55

Livello qualitativo

200

Forma fisica

liquid

impiego

sufficient for 1000 reactions
 sufficient for 250 reactions
 sufficient for 50 reactions

Caratteristiche

dNTPs included: no
hotstart: no

Concentrazione

1 unit/μL

tecniche

PCR: suitable

Colore

red

input

purified DNA

Compatibilità

suitable for PCR

applicazioni

agriculture

Condizioni di spedizione

wet ice

Temperatura di conservazione

−20°C

Descrizione generale

REDTaq® DNA Polymerase is a unique blend of Sigma′s quality Taq DNA Polymerase combined with an inert red dye. This dye enables quick visual confirmation of enzyme addition and reaction mixing. An aliquot of the samples (5-10 μl) can then be loaded directly onto an agarose gel for electrophoresis following PCR. The red dye migrates slightly faster than bromophenol blue at about the same rate as a 125 base pair fragment in a 1% agarose gel. Since no additional loading buffers are added to the reaction following PCR, reamplification is possible.
The red dye has no effect on automated or manual sequencing, restriction digestions or other downstream applications. However, if removing the dye is desired, this can easily be accomplished using any standard purification method.

Applicazioni

For routine PCR amplifications REDTaq® DNA Polymerase has been used in polymerase chain reaction (PCR) and reverse transcription-polymerase chain reaction (RT-PCR) analysis.

Caratteristiche e vantaggi

  • Same great performance as Taq DNA Polymerase in a more convenient format for high throughput applications.
  • Visual confirmation that not only has the enzyme been added, but that proper mixing has occurred.
  • No additional loading dyes are necessary. An aliquot can be taken directly from the reaction and loaded onto an agarose gel for electrophoresis.

Confezionamento

Provided with 10X reaction buffer containing MgCl2

Definizione di unità

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.

Note legali

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany

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Pittogrammi

Health hazard
GHS08

Avvertenze

Danger

Indicazioni di pericolo

H334

Consigli di prudenza

P261 - P284 - P501

Classi di pericolo

Resp. Sens. 1

Codice della classe di stoccaggio

10 - Combustible liquids

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

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Articoli

Introduction and Historical Timelines

Learn about the history of the polymerase chain reaction (PCR), from the basic principles that proceeded its discovery to the awarding of a Nobel Prize for Chemistry and more recent developments such as real-time PCR (qPCR) and digital PCR.

Protocolli

Applications with REDTaq™

Reviews the applications and benefits for RedTaq, including standard RedTaq, Hot Start RedTaq and RedTaq for genomic DNA PCR.

Standard PCR Protocol

Learn standard PCR protocol steps and review reagent lists or cycling parameters. This method for routine PCR amplification of DNA uses standard Taq DNA polymerase.

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