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S3401

Sigma-Aldrich

Sample Buffer, Laemmli 2× Concentrate

Sinonimo/i:

SDS Sample Buffer

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About This Item

Codice UNSPSC:
41105323
NACRES:
NA.25

Confezionamento

vial of 6 mL

Livello qualitativo

100
200

Temperatura di conservazione

−20°C

Applicazioni

Sample Buffer, Laemmli 2× concentrate has been used as a sample buffer for denaturing and loading of protein samples in SDS-PAGE.

Azioni biochim/fisiol

Laemmli sample buffer is especially formulated for protein sample preparation to be used in the Laemmli SDS-PAGE system. A protein sample is mixed with the 2X sample buffer (1:1) and heated in boiling water for 2-5 min. The 2-mercaptoethanol reduces the intra and inter-molecular disulfide bonds. The SDS detergent denatures the proteins and subunits and gives each an overall negative charge so that each will separate based on size. The bromophenol blue serves as a dye front that runs ahead of the proteins and also serves to make it easier to see the sample during loading. The glycerol increases the density of the sample so that it will layer in the sample well.

Altre note

Solution contains 4% SDS, 20% glycerol, 10% 2-mercaptoethanol, 0.004% bromphenol blue and 0.125 M Tris HCl, pH approx. 6.8.

Quantità

Recommended usage: add 1 volume gel loading solution to 1 volume sample and mix well.

Prodotti correlati

N° Catalogo
Descrizione
Determinazione del prezzo

92210

Timestrip Plus™ 0 °C

Avvertenze

Danger

Classi di pericolo

Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral

Codice della classe di stoccaggio

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

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Certificati d'analisi (COA)

Lot/Batch Number
SLCL3395
SLCJ2226
SLCF5270
SLCC3928
SLBZ7062

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Protocolli

Immunoprecipitation Procedure

To determine the molecular weights of protein antigens, to study protein/protein interactions, to determine specific enzymatic activity, to monitor protein post-translational modifications and to determine the presence and quantity of proteins.

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