D8906
Dextran sulfate sodium salt from Leuconostoc spp.
for molecular biology, average Mw >500,000 (dextran starting material), contains 0.5-2% phosphate buffer
Sinonimo/i:
6-O-(6-O-beta-D-Glucopyranosyl-beta-D-glucopyranosyl)-D-glucose, Manninotriose
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
About This Item
Prodotti consigliati
Origine biologica
synthetic
Livello qualitativo
Grado
for molecular biology
Forma fisica
powder
PM
average Mw >500,000 (dextran starting material)
contiene
0.5-2% phosphate buffer
Composizione
Sulfur Content, 16.0-19.0%
tecniche
FISH: suitable
FTIR: suitable
Colore
off-white
Intervallo di pH utile
6.0-8 (10 g/L)
Solubilità
H2O: 100 mg/mL
Compatibilità
suitable for marker for electrophoresis
suitable for nucleic acid hybridization
applicazioni
genomic analysis
sample preparation
Cerchi prodotti simili? Visita Guida al confronto tra prodotti
Descrizione generale
Dextran sulfate sodium (DSS) is a synthetic sulfated derivative of dextran, a complex polysaccharide composed of glucose molecules. DSS is characterized as a biocompatible, highly branched polysaccharide with 1–6 and 1–4 glycosidic linkages, featuring approximately 2.3 sulfate groups per glucosyl unit. Dextran sulfate is a sulfated polysaccharide with a variable molecular weight ranging from 5 to 1,400 kDa. It finds extensive use in molecular biology and biochemical research, with a particular emphasis on gastrointestinal investigations.DSS is widely recognized for its ability to induce colonic inflammation in mouse models of inflammatory bowel disease (IBD). It is widely recognized for its capacity to induce colonic inflammation in mouse models of inflammatory bowel disease (IBD) by creating models of acute, chronic, and relapsing IBD, by adjusting its concentration and administration frequency. Furthermore, Dextran sulfate is applied in various molecular biology techniques, such as precipitating lipoproteins, accelerating DNA probe hybridization, and facilitating ribosomal isolations. Notably, DSS serves as a valuable cross-linker for chitosan, allowing for the creation of microparticles and nanoparticles used in controlled drug delivery systems. Beyond these applications, DSS plays a central role in diverse research areas, particularly immunology and virology, where it actively modulates immune responses, enhances humoral immunity, influences B-lymphocyte activation, and affects thymocyte reactivity to lectins.
Applicazioni
Dextran sulfate has been used
- as a molecular weight marker to assess the purity and homogeneity of the purified polysaccharides by gel electrophoresis
- as a component of the hybridization buffer in fluorescence in situ hybridization (FISH) protocol for DNA detection and analysis
Dextran sulfate has many applications and when performing nucleic acid hybridizations, may be used as an accelerant to decrease the time required for probe annealing. Dextran sulfate may be included in the hybridization buffer when the rate of hybridization is a limiting factor in detecting rare sequences or it may be included in the hybridization buffer when a limited amount of probe is available. Dextran sulfate functions as an accelerant by excluding the nucleic acid from the volume of solution occupied by the polymer, thereby, increasing the effective probe concentration.
Azioni biochim/fisiol
Dextran sulfate, being a sulfated polysaccharide, consistently triggers experimental acute and chronic colitis. It is believed to initiate mucosal injury and inflammation, primarily through a direct toxic impact on epithelial cells. This initial effect activates macrophages and T lymphocytes, ultimately leading to cytokine-mediated cytotoxicity.
Caratteristiche e vantaggi
- Suitable for Nucleic acid hybridization & Molecular Biology
- Dextran Sulfate with an average molecular weight of >500,000
- Sulfur Content of 16.0-19.0 %
- Versatile and adaptable for wide variety of laboratory and research applications
Compatibilità
Tested for suitability in nucleic acid hybridizations
Altre note
For additional information on our range of Biochemicals, please complete this form.
To gain a comprehensive understanding of our extensive range of Dextrans for your research, we encourage you to visit our Carbohydrates Category page.
Prodotto comparabile
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
I clienti hanno visto anche
Identification of Wnt Genes Expressed in Neural Progenitor Zones during Zebrafish Brain Development.
Robert N Duncan et al.
PloS one, 10(12), e0145810-e0145810 (2015-12-30)
Wnt signaling regulates multiple aspects of vertebrate central nervous system (CNS) development, including neurogenesis. However, vertebrate genomes can contain up to 25 Wnt genes, the functions of which are poorly characterized partly due to redundancy in their expression. To identify
Benjamin R King et al.
Journal of virology, 92(12) (2018-04-13)
Lymphocytic choriomeningitis mammarenavirus (LCMV) is an enveloped, negative-strand RNA virus that causes serious disease in humans but establishes an asymptomatic, lifelong infection in reservoir rodents. Different models have been proposed to describe how arenaviruses regulate the replication and transcription of
Hiroki Ikeda et al.
Nature communications, 8(1), 1616-1616 (2017-11-22)
Higher-order chromatin organization controls transcriptional programs that govern cell properties and functions. In order for pluripotent stem cells (PSCs) to appropriately respond to differentiation signals, developmental gene loci should be structurally and spatially regulated to be readily available for immediate
Bhavana Tiwari et al.
Current biology : CB, 27(19), 3010-3016 (2017-10-03)
Retrotransposons are a pervasive class of mobile elements present in the genomes of virtually all forms of life [1, 2]. In metazoans, these are preferentially active in the germline, which, in turn, mounts defenses that restrain their activity [3, 4].
Danielle L Cullinane et al.
Reproduction (Cambridge, England), 149(1), 43-54 (2014-10-23)
The protamine 1 (Prm1) and sperm mitochondria-associated, cysteine-rich protein (Smcp) mRNAs exemplify a widespread pattern of mRNA-specific regulation of mRNA translation in post-meiotic spermatogenic cells, spermatids. Both mRNAs are transcribed and initially stored in free-mRNPs in early spermatids, and translated
Articoli
Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.
Protocolli
Dextran sulfates are supplied as the sodium salt forms, making them soluble and stable in water. Dextran sulfate contains approximately 17% sulfur which is equivalent to approximately 2.3 sulfate groups per glucosyl residue.
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica.