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Key Documents

M0644

Sigma-Aldrich

Terreno essenziale minimo di Eagle

Alpha Modification, with L-glutamine, ribonucleosides and deoxyribonucleosides, without sodium bicarbonate, powder, suitable for cell culture

Sinonimo/i:

MEM

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About This Item

Codice UNSPSC:
12352207
NACRES:
NA.75

Livello qualitativo

Forma fisica

powder

tecniche

cell culture | mammalian: suitable

Componenti

NaHCO3: no
L-glutamine: yes
phenol red: yes
HEPES: no
sodium pyruvate: yes

Condizioni di spedizione

ambient

Temperatura di conservazione

2-8°C

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Descrizione generale

Minimum Essential Medium (MEM) is a synthetic cell culture media developed by Harry Eagle. This medium has higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. Optional supplementation of non-essential amino acids to this formulation that incorporates either Hanks′ or Earle′s salts has broadened the usage of this medium. The α modification of MEM with Earle′s balanced salts also known as αMEM, contains non-essential amino acids, sodium pyruvate, and additional vitamins.

Applicazioni

Minimum essential medium Eagle has been used to:

  • maintain natural killer (NK) cell lines
  • culture Leishmania donovani strains
  • culture epidermal neural crest stem cells (EPI-NCSCs) from the bulge of rat hair follicles

Quantità

Formulated to contain 10.1 grams of powder per liter of medium.

Ricostituzione

Supplement with 2.2 g/L sodium bicarbonate.

Pittogrammi

Exclamation mark

Avvertenze

Warning

Indicazioni di pericolo

Classi di pericolo

Skin Sens. 1

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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Protocolli

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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