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M4526

Sigma-Aldrich

Terreno essenziale minimo di Eagle

Alpha Modification, with sodium bicarbonate and Earl's salts, without ʟ-glutamine, ribonucleosides and deoxyribonucleosides, liquid, sterile-filtered, suitable for cell culture

Sinonimo/i:

αMEM, MEM

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About This Item

Codice UNSPSC:
12352207
NACRES:
NA.71

product name

Terreno essenziale minimo di Eagle, Alpha Modification, with sodium bicarbonate, without L-glutamine, ribonucleosides and deoxyribonucleosides, liquid, sterile-filtered, suitable for cell culture

Livello qualitativo

400

Sterilità

sterile-filtered

Forma fisica

liquid

tecniche

cell culture | mammalian: suitable

Impurezze

endotoxin, tested

Componenti

Earle’s salts (5% CO2): yes
sodium pyruvate: yes
glucose: yes
L-glutamine: no
HEPES: no
Hanks’ salts (2% CO2): no
phenol red: yes
NaHCO3: yes
stable glutamine: no

Condizioni di spedizione

ambient

Temperatura di conservazione

2-8°C

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Categorie correlate

Descrizione generale

Minimum Essential Medium Eagle (MEM) is a synthetic cell culture media, developed by Harry Eagle. It has higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has enhanced the uses of this medium. The α modification of MEM with Earle′s balanced salts also known as αMEM, contains non-essential amino acids, sodium pyruvate, and additional vitamins.
This is the most enriched variation of the MEM formulation offered. It contains all 21 normal amino acids, some at increased concentrations. In addition, it contains 5 additional vitamins.

Applicazioni

Minimum Essential Medium Eagle has been used to maintain:
  • immature cumulus-oocyte complexes from mice
  • human mesenchymal stem cells (MSC)
  • mouse pre-osteoblastic cell line

Ricostituzione

Supplement with 0.292 g/L L-glutamine.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Michelle L Churchman et al.
Cancer cell, 33(5), 937-948 (2018-04-24)
Somatic genetic alterations of IKZF1, which encodes the lymphoid transcription factor IKAROS, are common in high-risk B-progenitor acute lymphoblastic leukemia (ALL) and are associated with poor prognosis. Such alterations result in the acquisition of stem cell-like features, overexpression of adhesion
Thi Thu Hien Bui et al.
Molecular reproduction and development, 84(5), 356-362 (2017-02-25)
Full-grown mouse antral oocytes are classified as surrounding nucleolus (SN) or not-surrounding nucleolus (NSN), depending on the respective presence or absence of a ring of Hoechst-positive chromatin surrounding the nucleolus. In culture, both types of oocytes resume meiosis and reach
Masaaki Kitada et al.
Glia, 67(5), 950-966 (2019-01-15)
Direct conversion is considered a promising approach to obtain tissue-specific cells for cell therapies; however, this strategy depends on exogenous gene expression that may cause undesired adverse effects such as tumorigenesis. By optimizing the Schwann cell induction system, which was
Ricardo Rodrigues-Pinto et al.
Scientific reports, 8(1), 12866-12866 (2018-08-29)
The adult nucleus pulposus originates from the embryonic notochord, but loss of notochordal cells with skeletal maturity in humans is thought to contribute to the onset of intervertebral disc degeneration. Thus, defining the phenotype of human embryonic/fetal notochordal cells is
Sarah Hemming et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 31(3), 1011-1027 (2016-12-10)
In this study, we examined the functional importance of EZH2 during skeletal development and homeostasis using the conditional deletion of
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